LOCUS       sequence-118711-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A carboxyl-terminal YFP fragment was fused to Ggamma-7. When 
            co-expressed with an amino-terminal YFP fragment fused to a Gbeta 
            subunit with which it interacts, a fluorescent signal is produced. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Hynes TR, Tang L, Mervine SM, Sabo JL, Yost EA, Devreotes PN, Berlot
            CH
  TITLE     Visualization of G protein betagamma dimers using bimolecular 
            fluorescence complementation demonstrates roles for both beta and 
            gamma in subcellular targeting.
  JOURNAL   J Biol Chem. 2004 Jul 16;279(29):30279-86. Epub 2004 May 10.
  PUBMED    15136579
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-07-16"; volume: "279(29)"; pages: "30279-86. 
            Epub 2004 May 10"
COMMENT     Sequence Label: YFP(159-238)-gamma-7 in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(268..289)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      471..480
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(511..530)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(512..530)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(575..778)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(604..624)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tgtgatagat ctttataaaa taatacaagg tttcttgtcc ttaaaggggt tctccgaggc
       61 agggactccg accagcaggg ggtcgttccg ggcatgttgc tcacagtagc tcatgaggtc
      121 agacgccgct ttggagacct tgatgcgctc aatcccggct tctatgcgta gctgttccac
      181 cagcttccgg gcctgggcta tgttgttagt ggctgacatg gatctcttgt acagctcgtc
      241 catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt
      301 ctcgttgggg tctttgctca gggcggactg gtagctcagg tagtggttgt cgggcagcag
      361 cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc
      421 gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttgc ccatggtggc
      481 ggatccgagc tcggtaccaa gcttgggtct ccctatagtg agtcgtatta atttcgataa
      541 gccagtaagc agtgggttct ctagttagcc agagagctct gcttatatag acctcccacc
      601 gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt
      661 cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa
      721 atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacc
//
