LOCUS       sequence-118755-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses UBE3C HECT domain in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Michel MA, Elliott PR, Swatek KN, Simicek M, Pruneda JN, Wagstaff 
            JL, Freund SM, Komander D
  TITLE     Assembly and specific recognition of k29- and k33-linked 
            polyubiquitin.
  JOURNAL   Mol Cell. 2015 Apr 2;58(1):95-109. doi: 
            10.1016/j.molcel.2015.01.042. Epub 2015 Mar 5.
  PUBMED    25752577
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.01.042"; journalName: "Mol Cell"; date: 
            "2015-04-2- 2"; volume: "58"; issue: "1"; pages: "95-109"
COMMENT     Sequence Label: pOPINS-UBE3C
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..35)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gttgcagccg gatctcagtg gtggtggtgg tggtgtttaa atggtctaga aagctttaag
       61 acagttcgaa gccagctgca cattcgatgg cgtacagcag cttgctgcgc agcagcgttt
      121 cgtcataaaa ttccggcagt ttcagcagat tcatgcacgt ggaagcggtc ggcagacgtt
      181 ccagatcact gccaccgttg tgaatacaga atgccggata cagttcctta aagcccagca
      241 gcggcgggcg agagcagctc gtcacgaatt tcagcagctt acgcttttct tcatcggtga
      301 aaccttccac gacgcgccaa aagactttga taaccggatg gtcggcggag tagccaccac
      361 tataattggt aaaagatttc aggtcttcca ggctaatcgg cacctgtgca ccggagatca
      421 gcacctgaat ttcttgctgg tcgaacatgc gcagccattc cagactaaca acgttggcca
      481 gaccctgacg aaacgccagg cagtgttgac ggatctggcg attcagacgg taatcagcaa
      541 ccagatggat ataggcaatg cggttcgcgc tcgtcaccgg aatgtccttg ccaccaaatt
      601 tcagttcgac aacctgtgct tcgcccagat cattgttcac gacggtgaaa ttcagaccca
      661 gttcttccac gtcatcttcg tacgacttca gaaacagcag gtttttatag acttccgggt
      721 ccaggctcgc caggtgatga atgtcaacat ctgccgaggt gcccagcagt tttgaca
//
