LOCUS       sequence-118790-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Const. Promoter ProD targetting RNA E. coli Low Phosphate Induction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  TITLE     SUPERSEDE
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCASCADE-proD
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     175..192
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(584..611)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(627..698)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(783..802)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa
       61 cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc ttccaggaaa
      121 tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga gcgtagcgag
      181 tcagtgagcg aggaagcgga atatatccct aggaaaaaaa accccgccct gtcaggggcg
      241 gggttttttt ttcggtttat ccccgctggc gcggggaact cgagcatgcc cgtaaagtta
      301 tccagcaacc actcggttta tccccgctgg cgcggggaac tcgaggtggt accagatctt
      361 ttgaattcaa agttaaacaa aattatttgt agagggaaac cgttgtggtc tccctgaata
      421 tacgcgtttt atgctcgcgg ggtaacatga catgctcgaa ttacagaaaa ataacttttt
      481 tgttacattt gtaagatagt aaggtgtcag aaagagttat ccagcaacca ctcatagacc
      541 tagggcagca gatagggacg acgtggtgtt agctgtgtat aaacgcagaa aggcccaccc
      601 gaaggtgagc cagtgtgact ctagtagaga gcgttcaccg acaaacaaca gataaaacga
      661 aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggatga tgtctagatt
      721 agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg
      781 tttaacaacc cgtaaactcg cccagaagct aggtgtagag cagcctacat tgtattgg
//
