LOCUS       sequence-118817-         665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TRABID NZF1-3 (aa 1-178) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Michel MA, Elliott PR, Swatek KN, Simicek M, Pruneda JN, Wagstaff 
            JL, Freund SM, Komander D
  TITLE     Assembly and specific recognition of k29- and k33-linked 
            polyubiquitin.
  JOURNAL   Mol Cell. 2015 Apr 2;58(1):95-109. doi: 
            10.1016/j.molcel.2015.01.042. Epub 2015 Mar 5.
  PUBMED    25752577
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.01.042"; journalName: "Mol Cell"; date: 
            "2015-04-2- 2"; volume: "58"; issue: "1"; pages: "95-109"
COMMENT     Sequence Label: pOPINK-TRABID (NZF1-3, aa 1-178)
FEATURES             Location/Qualifiers
     source          1..665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atggcacatc accatcacca tcacatgtcc
       61 cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa
      121 tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga
      181 aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat
      241 gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg
      301 ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt
      361 agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt
      421 cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat
      481 ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt
      541 ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt
      601 attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct
      661 ttgca
//
