LOCUS       sequence-118821-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella
            emersonii enables fast optical control of cGMP signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Scheib U, Stehfest K, Gee CE, Korschen HG, Fudim R, Oertner TG, 
            Hegemann P
  TITLE     The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella
            emersonii enables fast optical control of cGMP signaling.
  JOURNAL   Sci Signal. 2015 Aug 11;8(389):rs8. doi: 10.1126/scisignal.aab0611.
  PUBMED    26268609
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2015-08-11"; volume: "8(389):rs8. doi"; pages: " 
            10.1126/scisignal.aab0611"
COMMENT     Sequence Label: pAAV Syn hBE Rh Guanylyl Cyclase1 2A tDimer
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..673)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FL"
     primer_bind     complement(259..278)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     476..496
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     528..546
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 acaggaacag gtggtggcgg ccctcggagc gctcgtactg ttccacgatg gtgtagtcct
       61 cgttgtggga ggtgatgtcc agcttggtgt ccacgtagta gtagccgggc agctgcacgg
      121 gcttcttggc catgtagatg gtcttgaact ccaccaggta gtggccgccg tccttcagct
      181 tcagggcctg gtggatctcg cccttcagca cgccgtcgcg ggggtacagg cgctcggtgg
      241 aggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggggggg aagttggtgc
      301 cgcggaactt caccttgtag atcagcgtgc cgtcctgcag ggaggagtcc tgggtcacgg
      361 tcaccacgcc gccgtcctcg aagttcatca cgcgctccca cttgaagccc tcggggaagg
      421 acagcttctt gtagtcgggg atgtcggcgg ggtgcttcac gtacgccttg gagccgtact
      481 ggaactgggg ggacaggatg tcccaggcga agggcagggg gccgcccttg gtcaccttca
      541 gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact
      601 cgtggccgtt cacggagccc tccatgcgca ccttgaagcg catgaactct ttgatgacgt
      661 cctcggagga ggcggtgccg gagctgccgc tgccggtgct gccggtgcca tgccccagga
      721 acaggtggtg gcggccctcg gagcgctcgt actgttccac gatggtgtag tcctcgttgt
      781 gggaggtgat gtccagcttg gtgtccacgt agtagtagcc gggcagctgc acgggcttct
      841 tggccatgta gatggtcttg aactccacca ggtagtggc
//
