LOCUS       sequence-118898-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl8 with C-term GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARL8A-GFP
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    61..85
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(662..686)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(718..737)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(719..737)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(782..881)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(811..831)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 acaagaattg ggacaactcc agtgaaaagt tcttctcctt tgctagccat tctagatcga
       61 accactttgt acaagaaagc tgggtcgctt ctccgtgact tcgagtgttg aataagccac
      121 tgtagggtga tgtcaatgtt gtccttttct ttgcaagaga tggagtagca gcagatctct
      181 cggtcctgga tggcagacag attcattttc tcaatcagct ccttctcatc caatgctccc
      241 ggaaggtctc gcttgttacc caggactaag accgggatgc cctgcagctg aggtttgtcc
      301 agtaggttgt ggagctcgtt cttagaggcc tcaatcttct cctggtcagc agcatccacc
      361 atgtacacga tggcgctcac tcctcggcag tagcgctccc acatgctgcg gaaacgcggc
      421 tgtcccccaa tgtcccagag cttgatagtc acattccctt tggtgatttt gcgcatgttg
      481 aaacccacgg tggggatcat gtcctcgttg aactgtcctg acgcgatcac gttgacgaag
      541 gtggtcttgc ccgagtactg aagcccgacc agcgtgagct ccatctcctc cttccagaat
      601 agggccttga accagtccag cagcttgttg aacaaagcga tcatggttct atctccttct
      661 aagcctgctt ttttgtacaa acttgtttga tcaagcttaa ctagccagct tgggtctccc
      721 tatagtgagt cgtattaatt tcgataagcc agtaagcagt gggttctcta gttagccaga
      781 gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg
      841 cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat
      901 tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc a
//
