LOCUS       sequence-118902-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 CIRBP
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..28)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(171..190)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(172..190)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        222..326
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(545..564)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tgccgtggtg atggtggtga tggtggtgac cagaggtgcc catggtttca cttacgtgtt
       61 taatgtcttt acagacttta tacattactg tcatggttaa ataaaataaa ataaaataaa
      121 ataaaataaa ataaaataaa ataaaataaa ataaaataaa cttaagatgt ccctatagtg
      181 agtcgtatta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      241 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      301 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      361 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      421 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      481 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      541 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      601 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
      661 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
      721 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgc
//
