LOCUS       sequence-118909-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This vector attaches CFP(159-238) to the N-terminus of a protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Mervine SM, Yost EA, Sabo JL, Hynes TR, Berlot CH
  TITLE     Analysis of G protein betagamma dimer formation in live cells using 
            multicolor bimolecular fluorescence complementation demonstrates 
            preferences of beta1 for particular gamma subunits.
  JOURNAL   Mol Pharmacol. 2006 Jul;70(1):194-205. Epub 2006 Apr 26.
  PUBMED    16641313
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2006-07"; volume: "70(1)"; pages: "194-205. Epub
            2006 Apr 26"
COMMENT     Sequence Label: CFP(159-238) in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..61
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        42..60
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      92..101
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     283..304
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(396..414)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(397..414)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          498..563
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             693..713
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
       61 gagacccaag cttggtaccg agctcggatc cgccaccatg ggcaagaacg gcatcaaggc
      121 ccacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
      181 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
      241 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
      301 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagagat ctatcacact
      361 ggcggccgct cgagcatgca tctagagggc cctattctat agtgtcacct aaatgctaga
      421 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
      481 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
      541 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
      601 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
      661 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
      721 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
      781 c
//
