LOCUS       sequence-118913-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 YWHAB
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..35)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(173..192)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(174..192)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        224..328
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(547..566)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgcttgtgc cgtggtgatg gtggtgatgg tggtgaccag aggtgcccat ggtttcactt
       61 acgtgtttaa tgtctttaca gactttatac attactgtca tggttaaata aaataaaata
      121 aaataaaata aaataaaata aaataaaata aaataaaata aacttaagat gtccctatag
      181 tgagtcgtat tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      241 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      301 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      361 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      421 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      481 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      541 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      601 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      661 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      721 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg c
//
