LOCUS       sequence-118916-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This vector attaches Cerulean(1-158) to the N-terminus of a 
            protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Mervine SM, Yost EA, Sabo JL, Hynes TR, Berlot CH
  TITLE     Analysis of G protein betagamma dimer formation in live cells using 
            multicolor bimolecular fluorescence complementation demonstrates 
            preferences of beta1 for particular gamma subunits.
  JOURNAL   Mol Pharmacol. 2006 Jul;70(1):194-205. Epub 2006 Apr 26.
  PUBMED    16641313
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2006-07"; volume: "70(1)"; pages: "194-205. Epub
            2006 Apr 26"
COMMENT     Sequence Label: Cerulean(1-158) in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     171..190
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     430..451
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      493..502
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(533..552)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(534..552)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(597..800)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(626..646)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cggccgccag tgtgatagat ctctgcttgt cggcggtgat atagacgttg tcgctgatgg
       61 cgttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca
      121 gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt
      181 tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact
      241 tgaagaagtc gtgctgcttc atgtggtcgg ggtagcgggc gaagcactgc acgccccagg
      301 tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact
      361 tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt
      421 ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct
      481 cgcccttgct caccatggtg gcggatccga gctcggtacc aagcttgggt ctccctatag
      541 tgagtcgtat taatttcgat aagccagtaa gcagtgggtt ctctagttag ccagagagct
      601 ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag
      661 ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg
      721 tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg
      781 tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat gtactg
//
