LOCUS       sequence-118949-         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of native hArf4 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST14-ARF4
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(576..600)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(653..671)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      667..714
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aaaagcaggc ttagaaggag atagaaccat gggcctcact atctcctccc tcttctcccg
       61 actatttggc aagaagcaga tgcgcatttt gatggttgga ttggatgctg ctggcaagac
      121 aaccattctg tataaactga agttagggga gatagtcacc accattccta ccattggttt
      181 taatgtggaa acagtagaat ataagaacat ttgtttcaca gtatgggatg ttggtggtca
      241 agatagaatt aggcctctct ggaagcatta cttccagaat acccagggtc ttatttttgt
      301 ggtagatagc aacgatcgtg aaagaattca ggaagtagca gatgagctgc agaaaatgct
      361 tctggtagat gaattgagag atgcagtgct gctacttttt gcaaacaaac aggatttgcc
      421 aaatgctatg gccatcagtg aaatgacaga taaactaggg cttcagtctc ttcgtaacag
      481 aacatggtat gttcaagcca cttgtgcaac acaaggaact ggtctgtatg aaggacttga
      541 ctggctgtca aatgagcttt caaaacgtta ataggaccca gctttcttgt acaaagtggt
      601 gatgatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
      661 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
      721 ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat
      781 agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc
      841 tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata
      901 gtgactggcg atgctgtcgg aatggacgat atcccgcaag aaggcccggc agtaccgg
//
