LOCUS       sequence-118954-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of native hArl1 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST14-ARL1
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(580..604)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(657..675)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      671..718
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aaaaagcagg cttagaagga gatagaacca tgggtggctt tttctcaagt atattttcca
       61 gtctgtttgg aactcgggaa atgagaattt taattttggg attagatgga gcaggaaaaa
      121 ccacaatttt gtacagatta caagtgggag aagttgttac tactatacct accattggat
      181 ttaatgtaga gacggtgacg tacaaaaacc ttaaattcca agtctgggat ttaggaggac
      241 agacaagtat caggccatat tggagatgtt actattcaaa cacagatgca gtcatttatg
      301 tagtagacag ttgtgaccga gaccgaattg gcatttccaa atcagagtta gttgccatgt
      361 tggaggaaga agagctgaga aaagccattt tagtggtgtt tgcaaataaa caggacatgg
      421 aacaggccat gacttcctca gagatggcaa attcacttgg gttacctgcc ttgaaggacc
      481 gaaaatggca gatattcaaa acgtcagcaa ccaaaggcac cggccttgat gaggcaatgg
      541 aatggttagt tgaaacatta aaaagcagac agtaatagga cccagctttc ttgtacaaag
      601 tggtgatgat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
      661 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
      721 gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt
      781 cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggcc
//
