LOCUS       sequence-118973-         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of native hArl4D in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST14-ARL4D
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(640..664)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(717..735)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      731..778
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aaaaagcagg cttagaagga gatagaacca tggggaacca cttgactgag atggcgccca
       61 ctgcctcctc cttcttgccc cacttccaag ccctgcatgt cgtggtcatt gggctggact
      121 ctgctggaaa gacctccctc ctttaccgcc tcaagttcaa ggagtttgtc cagagtgtcc
      181 ccaccaaagg cttcaacacc gagaagatcc gggtgcccct cgggggatcg cgtggcatca
      241 ccttccaagt gtgggacgtc ggggggcagg agaagctgcg accactgtgg cgctcttata
      301 cccgccggac agacggtcta gtgtttgtgg tggacgctgc ggaggctgag cggctggagg
      361 aagccaaggt ggagttgcac cgaatcagcc gggcctcgga caaccagggc gtgccagtgc
      421 tggtgctggc caacaagcag gaccagcccg gggcactgag cgctgctgag gtggagaaga
      481 ggctggcagt ccgagagcta gcagccgcca ctctcactca tgtgcaaggc tgcagcgctg
      541 tggacggtct gggcctgcag cagggccttg agcgcctcta tgagatgatc ctcaagagga
      601 agaaggcagc tcggggtggc aagaagagac ggtgatagga cccagctttc ttgtacaaag
      661 tggtgatgat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
      721 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
      781 gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt
      841 cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca
      901 gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcac
//
