LOCUS       sequence-119007-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lim Lab plasmid: AmpR + PconShortNoHindM8::glmZ::Asp terminator + 
            PLlacO-1::T710 hfq::T1 terminator + PLtetO-1::glmS::gfp::T1T2 
            terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Sagawa S, Shin JE, Hussein R, Lim HN
  TITLE     Paradoxical suppression of small RNA activity at high Hfq 
            concentrations due to random-order binding.
  JOURNAL   Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
  PUBMED    26261213
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Aug 10. pii: gkv777."
COMMENT     Sequence Label: pHL2085
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(356..383)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(475..561)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     714..731
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 tacgattaaa gcttgggcct tcctgataca taaaaaaacg cctgctctta ttacggagca
       61 ggcgttaaaa caggtctgta tgacaacaag tgggtgcttc actcaacgtt gtgtccatgg
      121 tgtctgatga ggcataagcg acatctgtca gtggacgata agcaccgtaa acggctctgc
      181 gtcattccgg agtttatgag gcactaaggc gaacataaga gatggaatga gcatctaccc
      241 cgggaccata accgccaagc ataaaaatgt aaacaacgac ggtgctcgag gatcctgagc
      301 ggatacatat ttgaatgtat ttagaaaaat aaacaaaaga gtttgtagaa acgcaaaaag
      361 gccatccgtc aggatggcct tctgcttaat ttgatgcctg gcagtttatg gcgggcgtcc
      421 tgcccgccac cctccgggcc gttgcttcgc aacgttcaaa tccgctcccg gcggatttgt
      481 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga
      541 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcatgg ggagacccca
      601 cactaccatc ggcgctacgg cgtttcactt ctgagttcgg catggggtca ggtgggacca
      661 ccgcgctact gccgccaggc aaattctgtt ttatcagacc gcttctgcgt tctgatttaa
      721 tctgtatcag gctgaaaatc ttctctcatc cgccaaaaca gccaagctag cttttatttg
      781 tagagctcat ccatgccatg tgtaatccca gcagctgtta caaactcaag aagg
//
