LOCUS       sequence-119153-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RNA-binding protein MS2 with eDHFR fusion tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Carrocci TJ, Hoskins AA
  TITLE     Imaging of RNAs in live cells with spectrally diverse small molecule
            fluorophores.
  JOURNAL   Analyst. 2014 Jan 7;139(1):44-7. doi: 10.1039/c3an01550e. Epub 2013 
            Nov 1.
  PUBMED    24187681
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c3an01550e"; journalName: "Analyst"; date: "2014-01-7- 7"; 
            volume: "139"; issue: "1"; pages: "44-7"
COMMENT     Sequence Label: MS2-ecDHFR
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(12..655)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(96..119)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 tggtggatcc gtcgaaacta agttcttggt gttttaaaac taaaaaaaag actaactata
       61 aaagtagaat ttaagaagtt taagaaatag atttacagaa ttacaatcaa tacctaccgt
      121 ctttatatac ttattagtca agtaggggaa taatttcagg gaactggttt caaccttttt
      181 tttcagcttt ttccaaatca gagagagcag aaggtaatag aaggtgtaag aaaatgagat
      241 agatacatgc gtgggtcaat tgccttgtgt catcatttac tccaggcagg ttgcatcact
      301 ccattgaggt tgtgcccgtt ttttgcctgt ttgtgcccct gttctctgta gttgcgctaa
      361 gagaatggac ctatgaactg atggttggtg aagaaaacaa tattttggtg ctgggattct
      421 ttttttttct ggatgccagc ttaaaaagcg ggctccatta tatttagtgg atgccaggaa
      481 taaactgttc acccagacac ctacgatgtt atatattctg tgtaacccgc cccctatttt
      541 gggcatgtac gggttacagc agaattaaaa ggctaatttt ttgactaaat aaagttagga
      601 aaatcactac tattaattat ttacgtattc tttgaaatgg cagtattgat aatgataaac
      661 tcgacctgca gccaagctta tcgatgataa gctgtcaaac atgagaattc cacggactat
      721 agactatact agtatactcc gtctactgta cgatacactt ccgctcaggt ccttgtcctt
      781 taacgaggcc ttaccactct tttgttactc tattgatcca gctcagcaaa ggcagtgtga
      841 tctaagattc tatcttc
//
