LOCUS       sequence-119184-        1018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression plasmid for mouse Fli1; adds Flag tag to the N-terminus 
            of protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Roe JS, Mercan F, Rivera K, Pappin DJ, Vakoc CR
  TITLE     BET Bromodomain Inhibition Suppresses the Function of Hematopoietic 
            Transcription Factors in Acute Myeloid Leukemia.
  JOURNAL   Mol Cell. 2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011.
  PUBMED    25982114
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011."
COMMENT     Sequence Label: pcDNA3 Flag Fli1
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             120..143
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttggtaccga gctcggatct gccagcatgg
      121 actacaaaga cgatgacgac aaggacggga ctattaagga ggctctgtct gtggtgagtg
      181 acgatcagtc cctttttgat tcagcatacg gagcggcagc ccatctcccc aaggcagata
      241 tgactgcttc ggggagtcct gactacgggc agccccacaa aatcaacccc ctgccaccgc
      301 agcaggagtg gatcaaccag ccagtgagag tcaatgtcaa gcgggagtat gaccacatga
      361 atggatccag ggagtctccg gtggactgca gtgtcagcaa atgtaacaag ctggtgggcg
      421 gaggcgaagc caaccccatg aactataata gctacatgga tgagaagaac ggcccccctc
      481 ctcccaacat gaccaccaac gaacggagag tcattgtgcc tgcagacccc acactgtgga
      541 cacaggagca cgttcgacag tggctggagt gggctataaa ggaatacgga ttgatggaga
      601 ttgacacttc cttcttccag aacatggatg gcaaggaatt gtgtaaaatg aacaaggagg
      661 acttcctccg agccacctcc gcctacaaca cagaagtgct gttgtcgcac ctcagttacc
      721 tcagggaaag ttcactgctg gcctataaca caacctccca tacagaccag tcctcacgac
      781 tgaatgtcaa ggaagaccct tcttatgact ctgtcaggag aggagcatgg aacaataata
      841 tgaactctgg cctcaacaaa agtcctctcc ttggaggatc acagaccatg ggcaagaaca
      901 ctgagcagcg gccccagcca gatccttatc agatcctggg gccaaccagc agccgcctag
      961 caaaccctgg gagtgggcag atccagctgt ggcagtttct cctggaacta ctgtccga
//
