LOCUS       sequence-119247-         325 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  KIF1A aa 391-1698 with N-terminal FRB and triple myc tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Bentley M, Decker H, Luisi J, Banker G
  TITLE     A novel assay reveals preferential binding between Rabs, kinesins, 
            and specific endosomal subpopulations.
  JOURNAL   J Cell Biol. 2015 Feb 2;208(3):273-281. Epub 2015 Jan 26.
  PUBMED    25624392
REFERENCE   2  (bases 1 to 325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2015-02-2"; volume: "208(3)"; pages: "273-281. Epub 
            2015 Jan 26"
COMMENT     Sequence Label: pBa-FRB-3myc-KIF1A tail 391-1698
FEATURES             Location/Qualifiers
     source          1..325
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..325
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVXDLLXAWDLYYHVFRRIS"
ORIGIN
        1 ctccgcagcc cccaagcttg gcgcgcctag gcctaccatg gcttctagaa tcctctggca
       61 tgagatgtgg catgaaggcc tggaagaggc atctcgtttg tactttgggg aaaggaacgt
      121 gaaaggcatg tttgaggtgc tggagccctt gcatgctatg atggaacggg gcccccagac
      181 tctgaaggaa acatccttta atcaggccta tggtcgagat ttaatggagg cccaagagtg
      241 gtgcaggaag tacatgaaat cagggaatgt canggacctc ctccnagcct gggacctcta
      301 ttatcatgtg ttccgacgaa tctca
//
