LOCUS       sequence-119273-         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gRNA that targets stop codon for OCT4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Zhu Z, Verma N, Gonzalez F, Shi ZD, Huangfu D
  TITLE     A CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human 
            Embryonic Stem Cells.
  JOURNAL   Stem Cell Reports. 2015 May 28. pii: S2213-6711(15)00128-9. doi: 
            10.1016/j.stemcr.2015.04.016.
  PUBMED    26028531
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2015 May 28. pii: S2213-6711(15)00128-9. doi: 
            10.1016/j.stemcr.2015.04.016."
COMMENT     Sequence Label: Oct4-Cr1
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    34..133
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      158..167
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             167..232
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             239..259
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gaatcaccga cctctctccc cagggatccc ccccaaataa tgattttatt ttgactgata
       61 gtgacctgtt cgttgcaaca aattgatgag caatgctttt ttataatgcc aactttgtac
      121 aaaaaagcag gctccgcggt tttcaggttg gaccggtgcc accatggact ataaggacca
      181 cgacggagac tacaaggatc atgatattga ttacaaagac gatgacgata agatggcccc
      241 aaagaagaag cggaaggtcg gtatccacgg agtcccagca gccgacaaga agtacagcat
      301 cggcctggac atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt
      361 gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct
      421 gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac
      481 cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag
      541 caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt
      601 ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc
      661 ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga
      721 caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt tc
//
