LOCUS       sequence-119291-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes mCherry fused with an out-of-frame EGFP through a linker 
            containing a target for TALEN/CRISPR. Can be used as a surrogate 
            target to test the specificity of TALENs and CRISPR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Niccheri F, Pecori R, Conticello SG
  TITLE     An efficient method to enrich for knock-out and knock-in cellular 
            clones using the CRISPR/Cas9 system.
  JOURNAL   Cell Mol Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y.
  PUBMED    28421278
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y."
COMMENT     Sequence Label: pBS SK mCherryROSAegfp
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      362..371
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(368..370,371..373,374..832)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(413..434)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(674..693)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
       61 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
      121 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
      181 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagaagcttc
      241 gtctctgtcg gataatctgc aactccagtc tttccagaag atgggcggga gtcttctggg
      301 caaagatctg cgagacggaa ttctgcagtc gacggtaccg cgggcccggg atccaccggt
      361 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
      421 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      481 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
      541 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
      601 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      661 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      721 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      781 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
      841 gaagaacggc atcaaggtga acttcaag
//
