LOCUS       sequence-119320-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  hSar1 with stop codon in Gateway Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDONR221-SAR1
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..112
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(731..830)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tcagctggat ggcaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
       61 attgataagc aatgctttct tataatgcca actttgtaca agaaagctgg gtcctatcag
      121 tcaatatact gggagagcca gcggaaaccc tcgccgtaac cttgcctctt gagcacactg
      181 cacatgaaca cttccatggg gcgagcattc agctccttca gggtcacatt cccctttcct
      241 gtggtctgtc cataaagccc aaatatctca cggagttttt cttcactgat tgcatctgtt
      301 ctgtcaattt tgttacccaa gataaggatt ggcacattgg atattgtttc atcagtcatt
      361 aaagcattaa gctcaacttt ggattccacg aggcgagaat gatctgcaca gtccaccaga
      421 aagacaatcc cattaattgc tgggagataa tttttccaaa cgcgacgtgc ttgctcgtgc
      481 ccaccaagat caaaagttgt aaaggtcatt ccagcaattg ttagctcttc tgatgtcgga
      541 tgtagtgttg gaacatgttg gcccaatctg tcatctttga gcatgtgaag aagagtggtt
      601 ttgcctgcat tatccaaacc taagaataca agttttccag atttcttgta cagtcctagg
      661 aactggagca cactgctgaa gccattgtag atccactcaa agatgaaaga catggttcta
      721 tctccttcta agcctgcttt tttgtacaaa gttggcatta taaaaaagca ttgctcatca
      781 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg gggcccgagc
      841 ttaa
//
