LOCUS       sequence-119380-         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl1 with C-term Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDSHA-ARL1-Myc
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..49)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    63..87
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(649..673)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(784..802)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(848..899)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(877..897)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 attaaggtac cgggccctac agatcctctt cagagatgag tttctgctcc atctcgaatc
       61 gaaccacttt gtacaagaaa gctgggtcct gtctgctttt taatgtttca actaaccatt
      121 ccattgcctc atcaaggccg gtgcctttgg ttgctgacgt tttgaatatc tgccattttc
      181 ggtccttcaa ggcaggtaac ccaagtgaat ttgccatctc tgaggaagtc atggcctgtt
      241 ccatgtcctg tttatttgca aacaccacta aaatggcttt tctcagctct tcttcctcca
      301 acatggcaac taactctgat ttggaaatgc caattcggtc tcggtcacaa ctgtctacta
      361 cataaatgac tgcatctgtg tttgaatagt aacatctcca atatggcctg atacttgtct
      421 gtcctcctaa atcccagact tggaatttaa ggtttttgta cgtcaccgtc tctacattaa
      481 atccaatggt aggtatagta gtaacaactt ctcccacttg taatctgtac aaaattgtgg
      541 tttttcctgc tccatctaat cccaaaatta aaattctcat ttcccgagtt ccaaacagac
      601 tggaaaatat acttgagaaa aagccaccca tggttctatc tccttctaag cctgcttttt
      661 tgtacaaact tgtttgattc gagtgtcgac ggtatcgata agcttgatat cgaattcctg
      721 cagcccgggg gatccgcccg ggctagagcg gccgccaccg cggtggagct ccagcttttg
      781 ttccctttag tgagggttaa tttcgagctt ggcgtaatcg ctagcggatc tgacggttca
      841 ctaaaccagc tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca
      901 atggggcgga gttgttacga cattttggaa agtcccgttg aattttggtg ccaaaacaaa
      961 ctcccattga
//
