LOCUS       sequence-119415-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl2 with C-term V5 and His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST40-ARL2-V5-His6
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    95..119
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(690..714)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             740..781
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             791..808
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(831..848)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagttaagct atcaacaagt ttgtacaaaa aagcaggctt
      121 agaaggagat agaaccatgg ggctcctgac cattctgaag aagatgaagc agaaagagcg
      181 ggagctgcga ctgctcatgc ttggcctgga caatgctgga aagacaacca tcctgaagaa
      241 gttcaatggg gaggacatcg acaccatctc cccaacgctg ggcttcaaca tcaagaccct
      301 ggagcaccga ggattcaagc tgaacatctg ggatgtgggt ggccagaagt ccctgcggtc
      361 ctactggcgg aactactttg agagcaccga tggcctcatc tgggtagtgg acagcgcaga
      421 ccgccagcgc atgcaggact gccagcggga gctccagagc ctgctggtgg aggagcgcct
      481 ggccggagca accctcctca tctttgctaa taagcaggac ctgcctggag cactgtcctc
      541 taacgccatc cgcgaggccc tggagctgga ctccatccgc agccaccact ggtgcatcca
      601 gggctgcagc gccgtcaccg gggagaacct gctgccgggc atcgactggc tcctggatga
      661 catttccagc cgcattttca cagctgacga cccagctttc ttgtacaaag tggttgatct
      721 agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac
      781 gcgtaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag cctcgactgt
      841 gccttctagt tgccagccat ctgttgtt
//
