LOCUS       sequence-119531-       11291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Filifactor alocis Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11291)
  TITLE     Feng Zhang lab bacterial Cas9 orthologs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PX381 Filifactor alocis Cas9
FEATURES             Location/Qualifiers
     source          1..11291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     181..1008
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             181..305
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(343..948)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     enhancer        1086..1389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1390..1593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1544..1564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1615..1634
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1615..1633
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1634..1658
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        1661..1770
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    1772..1796
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1825..1851
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1855..1875
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6015..6032)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    complement(6037..6061)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    6063..6539
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(6262..6281)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     terminator      6540..6587
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6680..7108
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6767..6786)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6977..6998
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7117..7137)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7122..7451
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7302..7437
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7364..7383
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7518..8312
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7572..7591)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8182..8201
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8486..8607
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8523..8542)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8577..8596
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    8651..8706
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8705..8724)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     terminator      8794..8841
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_recomb     8854..9559
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           8942..9314
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(8971..9559)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     protein_bind    complement(9568..9601)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(9769..10356)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9848..9867)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10369..11160,11161..11229))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10992..11011
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
     1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
     1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
     1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
     1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
     1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
     1861 aaaaagcgca aggtcgaagc gtccatgacc aaggagtatt acctggggct ggatgtgggg
     1921 accaattccg tgggatgggc agtgaccgat tctcagtaca acctgtgcaa gtttaagaaa
     1981 aaggatatgt ggggcatccg gctgttcgaa agcgccaaca cagcaaagga ccggagactg
     2041 cagagaggga ataggcgccg actggagcgg aaaaagcaga gaattgatct gctgcaggaa
     2101 atcttctccc cagagatctg caagattgac cccactttct ttatccgact gaacgaatcc
     2161 cggctgcacc tggaggacaa gtctaacgat ttcaaatacc cactgtttat tgagaaggac
     2221 tattctgata tcgagtacta taaagagttc cccaccattt ttcacctgag gaagcatctg
     2281 atcgagagtg aggaaaaaca ggatatccgg ctgatctacc tggccctgca caacatcatt
     2341 aagacccgag gacattttct gattgacggc gatctgcaga gcgccaagca gctgaggccc
     2401 atcctggata cattcctgct gtccctgcag gaggaacaga acctgtcagt gagcctgtcc
     2461 gaaaatcaga aggacgagta tgaggaaatt ctgaaaaacc gcagcatcgc caagtccgaa
     2521 aaagtgaaaa agctgaagaa tctgtttgag atctcagacg agctggaaaa agaggagaag
     2581 aaggcccaga gcgccgtgat cgagaacttc tgcaagttta tcgtgggaaa taagggcgat
     2641 gtctgtaaat tcctgcgggt gtctaaggag gaactggaga ttgactcttt cagtttttca
     2701 gagggcaagt acgaggacga catcgtgaaa aacctggagg aaaaagtgcc tgaaaaggtc
     2761 tacctgtttg agcagatgaa ggcaatgtat gattggaata ttctggtcga catcctggaa
     2821 accgaggaat acatcagctt cgccaaagtg aagcagtatg agaaacacaa gactaacctg
     2881 cggctgctga gagacatcat tctgaaatac tgcaccaagg atgagtataa tcggatgttt
     2941 aacgacgaga aggaagctgg cagctacacc gcatatgtgg ggaaactgaa aaagaacaac
     3001 aagaagtact ggatcgagaa aaagagaaat cccgaggagt tctacaaatc cctgggcaag
     3061 ctgctggata aaattgagcc tctgaaggag gacctggaag tgctgactat gatgatcgag
     3121 gagtgtaaga accacaccct gctgccaatt cagaaaaata aggacaacgg cgtgatcccc
     3181 caccaggtgc atgaggtcga actgaaaaag atcctggaaa atgccaaaaa gtactattcc
     3241 ttcctgaccg agacagacaa ggatgggtac tcagtggtcc agaaaatcga gagcattttc
     3301 aggtttcgca tcccctacta tgtggggcct ctgagtaccc ggcaccagga aaagggatca
     3361 aacgtgtgga tggtcagaaa acctggcagg gaggatcgca tctacccatg gaatatggag
     3421 gaaatcattg actttgagaa gagcaacgaa aatttcatta cacggatgac taacaaatgt
     3481 acatatctga tcggggaaga tgtcctgccc aagcattctc tgctgtacag taaatatatg
     3541 gtgctgaatg agctgaacaa tgtgaaggtc agaggaaaaa agctgcctac atctctgaaa
     3601 cagaaggtgt tcgaggacct gtttgaaaac aaatccaaag tgactggaaa gaatctgctg
     3661 gagtacctgc agatccagga caaagatatc cagattgacg atctgtctgg cttcgacaag
     3721 gacttcaaga ccagcctgaa gagctatctg gacttcaaaa agcagatttt tggggaggaa
     3781 atcgagaagg aaagcattca gaacatgatc gaagatatca ttaagtggat caccatctac
     3841 ggcaatgaca aggagatgct gaaacgagtg attcgggcta attatagcaa ccagctgaca
     3901 gaggaacaga tgaaaaagat cactggattt cagtacagtg gctgggggaa cttctcaaag
     3961 atgtttctga aagggatcag cggatccgac gtgagcaccg gcgaaacatt cgacatcatt
     4021 accgcaatgt gggagacaga caacaatctg atgcagatcc tgtcaaaaaa gttcaccttt
     4081 atggacaacg tcgaggactt caacagcggc aaggtcggga aaatcgacaa gattacttac
     4141 gatagcaccg tgaaggaaat gttcctgtcc cctgagaaca aaagggccgt ctggcagacc
     4201 attcaggtgg ctgaggagat caagaaagtg atgggctgcg agccaaaaaa gatctttatt
     4261 gaaatggcac ggggcgggga gaaggtgaaa aagaggacaa aatctcgcaa ggcccagctg
     4321 ctggagctgt acgccgcttg cgaggaagat tgtagagaac tgatcaagga gattgaggac
     4381 cgggacgaga gggacttcaa tagcatgaag ctgtttctgt actataccca gttcgggaaa
     4441 tgtatgtatt ccggcgacga catcgatatt aacgagctga ttcgcggcaa ttctaagtgg
     4501 gaccgagatc acatctaccc ccagagcaaa attaaggacg attccatcga taacctggtg
     4561 ctggtcaata agacatataa tgccaaaaag tccaatgagc tgctgtctga ggacatccag
     4621 aaaaagatgc attcattctg gctgagcctg ctgaacaaaa agctgatcac taaaagcaag
     4681 tacgaccgcc tgactcgaaa gggcgacttt accgatgagg aactgagtgg gttcatcgct
     4741 agacagctgg tggaaacaag gcagtcaact aaggcaatcg ccgatatctt caagcagatc
     4801 tacagctccg aggtggtcta tgtgaagagc agcctggtga gcgacttcag gaaaaagcca
     4861 ctgaactacc tgaagtctcg gagagtcaat gattaccacc atgcaaaaga cgcctatctg
     4921 aacattgtgg tcgggaacgt gtacaacaaa aagtttacca gtaatcccat ccagtggatg
     4981 aaaaagaatc gcgatacaaa ctatagcctg aacaaggtgt tcgaacacga cgtggtcatt
     5041 aacggagaag tgatctggga aaagtgcaca taccatgagg acactaatac ctatgatgga
     5101 ggcactctgg accgaatccg gaagattgtg gaacgcgata acattctgta caccgagtac
     5161 gcttattgtg agaagggcga actgtttaat gcaaccatcc agaacaaaaa tggaaactcc
     5221 acagtctctc tgaaaaaggg cctggacgtg aaaaagtacg ggggatactt cagcgccaac
     5281 acaagttact tctcactgat cgagtttgag gacaagaagg gggatagagc aaggcacatc
     5341 attggagtgc ctatctatat tgcaaacatg ctggagcatt ctccaagtgc cttcctggag
     5401 tactgcgaac agaaggggta tcagaatgtg cggattctgg tcgagaaaat caaaaagaac
     5461 agcctgctga tcattaatgg ataccctctg cgcattcgag gcgagaacga agtggatact
     5521 tcctttaaga gggccatcca gctgaagctg gaccagaaaa actatgagct ggtccgcaat
     5581 atcgagaagt tcctggaaaa atacgtggag aaaaagggaa actatccaat tgacgagaat
     5641 agagatcaca tcacacatga aaagatgaac cagctgtacg aggtgctgct gtccaaaatg
     5701 aaaaagttca acaagaaggg catggccgac ccctctgata ggatcgaaaa gagtaagcct
     5761 aaattcatca agctggagga cctgatcgat aagattaatg tgatcaacaa aatgctgaac
     5821 ctgctgcgct gtgacaatga tactaaggcc gacctgtctc tgattgagct gcccaaaaac
     5881 gctgggagtt tcgtggtcaa aaagaatacc atcggaaagt caaaaatcat cctggtgaat
     5941 cagagcgtga ctggactgta cgagaataga cgggaactgt aagaattcgg taccgagctc
     6001 aagcttgcgg ccgcctatag tgtcacctaa atcgtaaccc agctttcttg tacaaagtgg
     6061 tcgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc
     6121 cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt
     6181 ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa gttgggatga
     6241 caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt
     6301 ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt
     6361 tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac
     6421 ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg atctacccac
     6481 cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc
     6541 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgtct agaagacaat
     6601 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     6661 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6721 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6781 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6841 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     6901 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6961 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     7021 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     7081 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     7141 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     7201 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7261 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     7321 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7381 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7441 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     7501 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     7561 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     7621 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     7681 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     7741 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     7801 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     7861 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     7921 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     7981 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     8041 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     8101 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     8161 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     8221 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     8281 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     8341 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     8401 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     8461 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     8521 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8581 tttgtccaaa ctcatcaatg tatcttatca tgtctgttca tgaagcccct tgagcatctg
     8641 acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt
     8701 ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg
     8761 gtttagagtt tggcaacata tgcccatatg taactagcat aaccccttgg ggcctctaaa
     8821 cgggtcttga ggggtttttt gctgaaagca tgcggaggaa attctccttg aagtttccct
     8881 ggtgttcaaa gtaaaggagt ttgcaccaga cgcacctctg ttcactggtc cggcgtatta
     8941 aaacacgata cattgttatt agtacattta ttaagcgcta gattctgtgc gttgttgatt
     9001 tacagacaat tgttgtacgt attttaataa ttcattaaat ttataatctt tagggtggta
     9061 tgttagagcg aaaatcaaat gattttcagc gtctttatat ctgaatttaa atattaaatc
     9121 ctcaatagat ttgtaaaata ggtttcgatt agtttcaaac aagggttgtt tttccgaacc
     9181 gatggctgga ctatctaatg gattttcgct caacgccaca aaacttgcca aatcttgtag
     9241 cagcaatcta gctttgtcga tattcgtttg tgttttgttt tgtaataaag gttcgacgtc
     9301 gttcaaaata ttatgcgctt ttgtatttct ttcatcactg tcgttagtgt acaattgact
     9361 cgacgtaaac acgttaaata gagcttggac atatttaaca tcgggcgtgt tagctttatt
     9421 aggccgatta tcgtcgtcgt cccaaccctc gtcgttagaa gttgcttccg aagacgattt
     9481 tgccatagcc acacgacgcc tattaattgt gtcggctaac acgtccgcga tcaaatttgt
     9541 agttgagctt tttggaattg cgatcgcata acttcgtata gcatacatta tacgaagtta
     9601 taagctcgga acgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     9661 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     9721 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     9781 ccgccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     9841 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     9901 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     9961 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    10021 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    10081 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    10141 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    10201 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    10261 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    10321 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgtt accaatgctt
    10381 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    10441 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    10501 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    10561 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    10621 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10681 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10741 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10801 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10861 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    10921 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    10981 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    11041 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    11101 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    11161 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    11221 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    11281 gtccgcgcgt t
//
