LOCUS       sequence-119536-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hSar1 with C-term V5 and His6 tag in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pET-DEST42-SAR1-V5-His6
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    41..65
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(678..702)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             722..763
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             773..790
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(848..866)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ccctctagaa taattttgtt taactttaag aaggaatatc acaagtttgt acaaaaaagc
       61 aggcttagaa ggagatagaa ccatgtcttt catctttgag tggatctaca atggcttcag
      121 cagtgtgctc cagttcctag gactgtacaa gaaatctgga aaacttgtat tcttaggttt
      181 ggataatgca ggcaaaacca ctcttcttca catgctcaaa gatgacagat tgggccaaca
      241 tgttccaaca ctacatccga catcagaaga gctaacaatt gctggaatga cctttacaac
      301 ttttgatctt ggtgggcacg agcaagcacg tcgcgtttgg aaaaattatc tcccagcaat
      361 taatgggatt gtctttctgg tggactgtgc agatcattct cgcctcgtgg aatccaaagt
      421 tgagcttaat gctttaatga ctgatgaaac aatatccaat gtgccaatcc ttatcttggg
      481 taacaaaatt gacagaacag atgcaatcag tgaagaaaaa ctccgtgaga tatttgggct
      541 ttatggacag accacaggaa aggggaatgt gaccctgaag gagctgaatg ctcgccccat
      601 ggaagtgttc atgtgcagtg tgctcaagag gcaaggttac ggcgagggtt tccgctggct
      661 ctcccagtat attgacgacc cagctttctt gtacaaagtg gtgatcaatt cgaagcttga
      721 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca
      781 ccatcaccat tgagtttgat ccggctgcta acaaagcccg aaaaggaagc tgagttggct
      841 gctgccaccg ctgagcaata actagcataa ccccttgggg
//
