LOCUS       sequence-119571-        1082 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 E-Cad
FEATURES             Location/Qualifiers
     source          1..1082
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    176..200
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(648..672)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(673..696)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(739..760)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttctgcaggg taccacgcgt acccaacaca cggacacatg cacgaacacg catgcagaga
       61 gatcatacat catctataga gaagtacacg taaaagtaag actcgtcgtc tgccagctac
      121 aaccgagaag gcgacgtgaa aggaacaaga aaggaggagg agggcggccg cttacaccac
      181 tttgtacaag aaagctgggt tgtcgtcctc gccgcctccg tacatgtcag ccagcttctt
      241 gaagcgattg ccccattcgt tcaagtagtc atagtcctgg tctttgtctg actctgagga
      301 gttcagggag ctcagactag cagcttcgga accgcttcct tcatagtcaa acacgagcag
      361 agaatcataa ggcggggctg tggggtcagt atcagccgct ttcagatttt catcaataaa
      421 atttccaatt tcatcgggat tggcagggcg gggaagatac cgggggacac tcatgagggt
      481 tggtgcaacg tcgttacgag tcacttcagg ccgagcgtcc aggcccctgt gcagctggct
      541 caagtcaaag tcctggtcct cttctccgcc tccttcttca tcatagtaat aaacgttgtc
      601 ccgggtgtca tcctctgggg gcagtaaggg ctctttgacc accgcgaagc ctgctttttt
      661 gtacaaactt gtcggaccct ggaagagcac ctccagcttg tatagctcgt ccatgccgag
      721 agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg
      781 gtctttgctc agggcggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc
      841 gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat
      901 gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata
      961 gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt
     1021 gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga acttccctcg
     1081 gc
//
