LOCUS       sequence-119572-        1018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl8 with C-term V5 and His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST40-ARL8A-V5-His6
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(589..613)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             639..680
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             690..707
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(730..747)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    736..961
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 aaaaagcagg cttagaagga gatagaacca tgatcgcttt gttcaacaag ctgctggact
       61 ggttcaaggc cctattctgg aaggaggaga tggagctcac gctggtcggg cttcagtact
      121 cgggcaagac caccttcgtc aacgtgatcg cgtcaggaca gttcaacgag gacatgatcc
      181 ccaccgtggg tttcaacatg cgcaaaatca ccaaagggaa tgtgactatc aagctctggg
      241 acattggggg acagccgcgt ttccgcagca tgtgggagcg ctactgccga ggagtgagcg
      301 ccatcgtgta catggtggat gctgctgacc aggagaagat tgaggcctct aagaacgagc
      361 tccacaacct actggacaaa cctcagctgc agggcatccc ggtcttagtc ctgggtaaca
      421 agcgagacct tccgggagca ttggatgaga aggagctgat tgagaaaatg aatctgtctg
      481 ccatccagga ccgagagatc tgctgctact ccatctcttg caaagaaaag gacaacattg
      541 acatcaccct acagtggctt attcaacact cgaagtcacg gagaagcgac ccagctttct
      601 tgtacaaagt ggttgatcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc
      661 tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc
      721 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
      781 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
      841 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
      901 gcaaggggga aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
      961 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgta
//
