LOCUS       sequence-119579-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hSara with C-term V5 and His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST40-SARA-V5-His6
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(625..649)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             675..716
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             726..743
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(766..783)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    772..883
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 aaaaagcagg cttagaagga gatagaacca tgtccttcat atttgattgg atttacagtg
       61 gtttcagcag tgtgctacag tttttaggat tatataagaa aactggtaaa ctggtatttc
      121 ttggattgga taatgcagga aaaacaacat tgctacacat gctaaaagat gacagacttg
      181 gacaacatgt cccaacatta catcccactt ccgaagaact gaccattgct ggcatgacgt
      241 ttacaacttt tgatctgggt ggacatgttc aagctcgaag agtgtggaaa aactaccttc
      301 ctgctatcaa tggcattgta tttctggtgg attgtgcaga ccacgaaagg ctgttagagt
      361 caaaagaaga acttgattca ctaatgacag atgaaaccat tgctaatgtg cctatactga
      421 ttcttgggaa taagatcgac agacctgaag ccatcagtga agagaggttg cgagagatgt
      481 ttggtttata tggtcagaca acaggaaagg ggagtatatc tctgaaagaa ctgaatgccc
      541 gacccttaga agttttcatg tgtagtgtgc tcaaaagaca aggttacgga gaaggcttcc
      601 gctggatggc acagtacatt gatgacccag ctttcttgta caaagtggtt gatctagagg
      661 gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta
      721 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt
      781 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
      841 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
      901 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggag
//
