LOCUS       sequence-119594-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targets HA-eGFP to mitochondrial matrix.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pCMV4-OCT-HA-eGFP
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..43)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(204..223)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(205..223)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(268..319)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(297..317)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     533..552
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        627..731
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccttgctcac gaattcagcg taatctggaa catcgtatgg gtaggatccc tggactggct
       61 tcccataccg aaaatttcga accatggaag tgtgagcctt tctaagagct gccttgttga
      121 gcaggattct caaattagac agcatggtac ctccggatcg ataattctag agggcccgtt
      181 taaacgctag ccagcttggg tctccctata gtgagtcgta ttaatttcga taagccagta
      241 agcagtgggt tctctagtta gccagagagc tctgcttata tagacctccc accgtacacg
      301 cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg
      361 atacgcgtat atctggcccg tacatcgcga agcagcgcaa aacgcctaac cctaagcaga
      421 ttcttcatgc aattgtcggt caagccttgc cttgttgtag cttaaatttt gctcgcgcac
      481 tactcagcga cctccaacac acaagcaggg agcagatact ggcttaacta tgcggcatca
      541 gagcagattg tactgagagt gcaccatagg ggatcgggag atctcccgat ccgtcgacgt
      601 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      661 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      721 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      781 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      841 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atcc
//
