LOCUS       sequence-119630-        1084 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 GTF2B
FEATURES             Location/Qualifiers
     source          1..1084
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    166..190
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taccacgcgt acccaacaca cggacacatg cacgaacacg catgcagaga gatcatacat
       61 catctataga gaagtacacg taaaagtaag actcgtcgtc tgccagctac aaccgagaag
      121 gcgacgtgaa aggaacaaga aaggaggagg agggcggccg cttacaccac tttgtacaag
      181 aaagttgggt atagctgtgg tagtttgtcc actggggtgt caaatttgaa gtctgtagga
      241 aacagatctg gggctcgagg atagatcagt ctataggact gtctgattgt aacatcagca
      301 acaccagcaa tatctccaat ttctttttgg gtcctctttt cagctgatgc ctgtgaggcc
      361 atgtaaatag ctgccgctgc cacagagatg gggctcctcc caggaaccaa gtccaattcc
      421 acagctttac gggctatatg tgtagctgcc atctgtactt gtttaggaag acaaaggttg
      481 gaacagaacc tggacatgaa gtccccagtt gtaatcaaat ccacactggt ttctagcgct
      541 ttcaaaataa gtttaaaaca ccgaccaatt tctttcttag aaattcgtga tacggcacat
      601 atttctttaa atgtcctagg aaccccttct tgtctacagg caatatagag acaagcagaa
      661 gctatagcat cattagctct tcccttcagg ctcttctgtt catatacttg cttgaataaa
      721 ttatttgttc gatcaactat atttcgaggt agattgattc tgtctgccat ggtagtgatt
      781 tctttgaatg cattcatcat tgcccgatca gaactgctca ttgttctccg attctggtac
      841 ttagaattgc caaattcgtc aaaacttgca gctcctgtgc ccttgccaat catggtagac
      901 aaatctccat cactcagaag aggattctga gaatctccaa ctcgagatgg atcttttgtt
      961 gctttgtcat tgctgaaagt tcgccattca gatcccacat caataacccg gtcacctaca
     1021 accaagccac attcaggaca gatcatatca ccggctctgt agtcctccac taaaatcgca
     1081 tctg
//
