LOCUS       sequence-119642-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 RUVBL1
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    174..198
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctgcagggta ccacgcgtac ccaacacacg gacacatgca cgaacacgca tgcagagaga
       61 tcatacatca tctatagaga agtacacgta aaagtaagac tcgtcgtctg ccagctacaa
      121 ccgagaaggc gacgtgaaag gaacaagaaa ggaggaggag ggcggccgct tacaccactt
      181 tgtacaagaa agttgggcac ttcatgtact tatcctgctg gtcagccagg attttggcgg
      241 aggacttggc atcatagaaa agttcactga tctcttcgac atgctctttc tcaatgctgt
      301 ccttcccgtt gattttagca agcaagttgg ccggggtcag cagctgcact gagtacctca
      361 gtgtggtctt ggtgccaatc tcccccaggt ggttcagtgc ctcctcactg atgttgattc
      421 cttccgtctg ggcacggatt ttaatgatct gtttcatttc ctgtggagta tacagcatgg
      481 tccggattat catcactcgg tccagaaggt caagagggat gccgtgaggg gatgtgatgt
      541 cctcagtgcc tctgatgaca cagttgcctc ggttggatgc aaagatgacg atgggagcga
      601 tagaagactc cagggcgcgg tgcaggtagg tgaagcactc aatgtccagc atgtggacct
      661 catcaacaaa cagcacaccc gggaccagct cagcaatgcc ctggtcgatg tacttgttca
      721 ccaccttatt aatctcccct cgaagtttgt ctgtgatttc tgtcttcttt ggcttcatta
      781 gctggcccat catggacagg atatcttgtc ccccctgggg ccgcgcatta gccacatcca
      841 agtcatgcaa ggtcacatct tggatgattt ctttcttttt gtgcacatcc ccttttggca
      901 aggggacata ctcttcagct tcaaggtcga attctgtggc ataggtatca cacctgccct
      961 gcctcttcac ggccccactg ttggcttcaa tgtaaatcac atctccagct tctactcgct
     1021 ctttctgcaa actttcaaaa
//
