LOCUS       sequence-119684-        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A 
            mutations. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B,
            Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F
  TITLE     In vivo genome editing using Staphylococcus aureus Cas9.
  JOURNAL   Nature. 2015 Apr 1. doi: 10.1038/nature14299.
  PUBMED    25830891
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Apr 1. doi: 10.1038/nature14299."
COMMENT     Sequence Label: 
            pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             47..67
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctatataagc agagctctct ggctaactac cggtgccacc atggccccaa agaagaagcg
       61 gaaggtcggt atccacggag tcccagcagc caagcggaac tacatcctgg gcctggccat
      121 cggcatcacc agcgtgggct acggcatcat cgactacgag acacgggacg tgatcgatgc
      181 cggcgtgcgg ctgttcaaag aggccaacgt ggaaaacaac gagggcaggc ggagcaagag
      241 aggcgccaga aggctgaagc ggcggaggcg gcatagaatc cagagagtga agaagctgct
      301 gttcgactac aacctgctga ccgaccacag cgagctgagc ggcatcaacc cctacgaggc
      361 cagagtgaag ggcctgagcc agaagctgag cgaggaagag ttctctgccg ccctgctgca
      421 cctggccaag agaagaggcg tgcacaacgt gaacgaggtg gaagaggaca ccggcaacga
      481 gctgtccacc aaagagcaga tcagccggaa cagcaaggcc ctggaagaga aatacgtggc
      541 cgaactgcag ctggaacggc tgaagaaaga cggcgaagtg cggggcagca tcaacagatt
      601 caagaccagc gactacgtga aagaagccaa acagctgctg aaggtgcaga aggcctacca
      661 ccagctggac cagagcttca tcgacaccta catcgacctg ctggaaaccc ggcggaccta
      721 ctatgaggga cctggcgagg gcagcccctt cggctggaag gacatcaaag aatggtacga
      781 gatgctgatg ggccactgca cctacttccc cgaggaactg cggagcgtga agtacgccta
      841 caacgccgac ctgtacaacg ccctgaacga cctgaacaat ctcgtgatca ccagggacga
      901 gaacgagaag ctggaatatt acgagaagtt ccagatcatc gagaacgtgt tcaagcagaa
      961 gaagaagccc accctgaagc agatcgccaa agaaatcctc gtgaacgaag aggatattaa
     1021 gggctacaga gtgaccagca ccggcaagcc cgagttc
//
