LOCUS       sequence-119685-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 SMAD2
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..42)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(185..204)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(186..204)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        236..340
                     /gene="bla"
                     /label=AmpR promoter
     CDS             525..903
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(559..578)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctcgcccttg cttgtgccgt ggtgatggtg gtgatggtgg tgaccagagg tgcccatggt
       61 ttcacttacg tgtttaatgt ctttacagac tttatacatt actgtcatgg ttaaataaaa
      121 taaaataaaa taaaataaaa taaaataaaa taaaataaaa taaaataaaa taaacttaag
      181 atgtccctat agtgagtcgt attagacgtc aggtggcact tttcggggaa atgtgcgcgg
      241 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
      301 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
      361 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
      421 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
      481 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
      541 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
      601 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
      661 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
      721 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
      781 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
      841 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
      901 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
      961 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
//
