LOCUS       sequence-119696-         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 TFEB
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    167..191
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taccacgccg tacccaacac acggacacat gcacgaacac gcatgcagag agatcataca
       61 tcatctatag agaagtacac gtaaaagtaa gactcgtcgt ctgccagcta caaccgagaa
      121 ggcgacgtga aaggaacaag aaaggaggag gagggcggcc gcttacacca ctttgtacaa
      181 gaaagttggg cacagcacat cgccctcctc catgctgaag ctgctccggc ggctgctggc
      241 cttggaggcc tcgggggaca tggtggacag aagtggatca gaggccagcg gtagcagtga
      301 gtcgtccagg agcatgaggt ccagatcctt cttggacagg ctggggaatg gggagccatg
      361 ccccggcgcc aggggttcgg ggtagcccgg gggaccctcg tcctccctgc ccccaaagct
      421 caggctgtgg ctgaagtcca ggtgatggaa tggggatggt ggctgggtgg gcaggggcag
      481 cggggcttgc gggggcagag ctggcagtgg ctcagggtca gggacctcag cccccagcat
      541 cagggcctcc cctgggccct cttcgctagg cagctcctgc ttcaccacct gctgggccag
      601 ctcagccatg ttcatgccgg acggggaggt ggtagggagg ccgtgcactc gagcctgcat
      661 ctccagctcc tggatacgga gccagagctg cttgttggtc atctccaggc ggcgagagtg
      721 gttctccagc tccctggact tttgcaggtc cttctgcatc ctccggatgt aatccacaga
      781 ggccttgagg atggtgccct tgttccagcg cacgtccagg tcattggcct tggggatcag
      841 cattcccaac tccttgatgc ggtcattgat gttgaacctt cgtctccttt caattaagtt
      901 gtgattgtct ttcttctgcc gctccttggc cagggccctg ctctcagcat ctgtgagctc
      961 tcgcttctgg gtcaggtccg cagggca
//
