LOCUS       sequence-119745-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Voltage sensing domain of Ciona with the D136A/A154D/E183N/R223Q 
            mutations fused to super ecliptic pHlorin A227D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Piao HH, Rajakumar D, Kang BE, Kim EH, Baker BJ
  TITLE     Combinatorial mutagenesis of the voltage-sensing domain enables the 
            optical resolution of action potentials firing at 60 Hz by a 
            genetically encoded fluorescent sensor of membrane potential.
  JOURNAL   J Neurosci. 2015 Jan 7;35(1):372-85. doi: 
            10.1523/JNEUROSCI.3008-14.2015.
  PUBMED    25568129
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.3008-14"; journalName: "J Neurosci"; date: 
            "2015-01-7- 7"; volume: "35"; issue: "1"; pages: "372-85"
COMMENT     Sequence Label: CC2
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      822..831
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(850..869)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(851..869)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(914..1013)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(943..963)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atctaattca acaagaattg ggacaactcc agtgaaaagt tcttctcctt tactcatgcg
       61 atatcttctt ttgttctgtg atattgttct tctgcttgaa gccttcattt gttggtggga
      121 ataaaatatt cttgctaacc gaaccacacg cagcaactgg gccaacacaa ccaaccgccc
      181 caaaccatcg gctcctgttt cggtaccgta ttcatctaac acagtgtaaa atatcgtgac
      241 gacgaatgtg acaacgataa tcaaaccatc agccacgttc caagggttgg tgaagaaaag
      301 cttgggcccg taggcaaata tccttaatcc taaatccagc atgaaataac aagaaagagc
      361 caagtccatc ccgtcataaa aggattgtga agattcactt ttatcgataa gactgagggc
      421 aatgatcatg aggatgatgt ccaagaaaat taggaagact ccaaagactc gcatccctag
      481 atgatcaatc actgctcgga tccgaaactg gacgcgacct acaccagtag taggtatctc
      541 cattctccca tcgtccactc catgttcatt ctcgccccac catagacctg aaatctcggg
      601 tatatctatt cgttcctcct gtcttgtctc tggtttttca ctcggtcctt ggtctacatc
      661 attccaatga actttcttta ccgattcact ttttcttggc gcagctttcg gcggagcagt
      721 gacgtcacca tttatcgtga cgtcaacgac gttccgcatg actgcaccgt caacgccaac
      781 taaatcagct ggaggactaa aatctgaacc gtcgaatccc tccatggtgg cgctagccag
      841 cttgggtctc cctatagtga gtcgtattaa tttcgataag ccagtaagca gtgggttctc
      901 tagttagcca gagagctctg cttatataga cctcccaccg tacacgccta ccgcccattt
      961 gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa
     1021 acaaactccc attgacgtca atggggtgga gacttggaaa tc
//
