LOCUS       sequence-119747-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Voltage sensing domain of Ciona with the D129S/R168A/D186S/R229I 
            mutations fused to super ecliptic pHlorin A227D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Piao HH, Rajakumar D, Kang BE, Kim EH, Baker BJ
  TITLE     Combinatorial mutagenesis of the voltage-sensing domain enables the 
            optical resolution of action potentials firing at 60 Hz by a 
            genetically encoded fluorescent sensor of membrane potential.
  JOURNAL   J Neurosci. 2015 Jan 7;35(1):372-85. doi: 
            10.1523/JNEUROSCI.3008-14.2015.
  PUBMED    25568129
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.3008-14"; journalName: "J Neurosci"; date: 
            "2015-01-7- 7"; volume: "35"; issue: "1"; pages: "372-85"
COMMENT     Sequence Label: CC4
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..81
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gccttactgg cttatcgaaa
       61 ttaatacgac tcactatagg gagacccaag ctggctagcg ccaccatgga gggattcgac
      121 ggttcagatt ttagtcctcc agctgattta gttggcgttg acggtgcagt catgcggaac
      181 gtcgttgacg tcacgataaa tggtgacgtc actgctccgc cgaaagctgc gccaagaaaa
      241 agtgaatcgg taaagaaagt tcattggaat gatgtagacc aaggaccgag tgaaaaacca
      301 gagacaagac aggaggaacg aatagatata cccgagattt caggtctatg gtggggcgag
      361 aatgaacatg gagtggacga tgggagaatg gagataccta ctactggtgt aggtcgcgtc
      421 cagtttcgga tccgagcagt gattgatcat ctagggatgc gagtctttgg agtcttccta
      481 attttcttga gcatcatcct catgatcatt gatctcagtc ttatcgataa aagtgaatct
      541 tcacaatcct tttatgacgg gatggccttg gctctttctt gttatttcat gctggactta
      601 ggattagcca tatttgccta cgggcccaag cttttcttca ccaacccctg ggaggttgct
      661 tccggtttga ttatcgttgt cacattcgtc gtcacgatat tttacactgt gttagatgaa
      721 tacggtaccg aaacaggagc cgatggtttg gggcggttgg ttgtgttggc cagattgctg
      781 cgtgtggtta tcttagcaag aatattttat tcccaccaac aaatgaaggc ttcaagcaga
      841 agaacaatat cacagaacaa aagaagatat cgcatgagta aaggagaaga actttt
//
