LOCUS       sequence-119842-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 IL2
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..39
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    40..64
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     sig_peptide     64..123
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     protein_bind    complement(524..548)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      772..818
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gacgagctat acaagctgga ggtgctcttc cagggtccga caagtttgta caaaaaagtt
       61 ggcatgtaca ggatgcaact cctgtcttgc attgcactaa gtcttgcact tgtcacaaac
      121 agtgcaccta cttcaagttc tacaaagaaa acacagctac aactggagca tttactgctg
      181 gatttacaga tgattttgaa tggaattaat aattacaaga atcccaaact caccaggatg
      241 ctcacattta agttttacat gcccaagaag gccacagaac tgaaacatct tcagtgtcta
      301 gaagaagaac tcaaacctct ggaggaagtg ctaaatttag ctcaaagcaa aaactttcac
      361 ttaagaccca gggacttaat cagcaatatc aacgtaatag ttctggaact aaagggatct
      421 gaaacaacat tcatgtgtga atatgctgat gagacagcaa ccattgtaga atttctgaac
      481 agatggatta ccttttgtca aagcatcatc tcaacactga cttgcccaac tttcttgtac
      541 aaagtggtgt aagcggccgc cctcctcctc ctttcttgtt cctttcacgt cgccttctcg
      601 gttgtagctg gcagacgacg agtcttactt ttacgtgtac ttctctatag atgatgtatg
      661 atctctctgc atgcgtgttc gtgcatgtgt ccgtgtgttg ggtacgcgtg gtaccctgca
      721 ggaaggaagc tgagttggct gctgccaccg ctgagcaata actagtaatt actagcataa
      781 ccccttgggg cctctaaacg ggtcttgagg gggttttttg ctgaaaggag gacagctgat
      841 gattgtcatg cttgccatct gttttcttg
//
