LOCUS       sequence-120000-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 CIRBP
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(709..733)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(734..757)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(800..821)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gtactcgttg tgtgtagcgt aactgtcgta actgtctctg taggacccgc
      241 ccgagctccg gtcactgtag ccaccactct gactccggct gctatagtag tctctggagc
      301 ctccgtagcc cccactcctg gactcgaacc ggttcccccc atagcctcgg tcccctcctc
      361 ctctagagaa cccacggccc cgtcctcggc ccccacggaa gaagccccgg cccccggcag
      421 agccaccacg gtacccacgg gatcggttgt ctgacgactt gcctgcctgg tctactcgga
      481 tctgccgtcc atctacagac ttcccattca tggccatcat ggcatcctta gcgtcgtcaa
      541 tgttctcaaa ggtgacaaac ccaaatcccc gagatctctg ggtctccctg tctttcacaa
      601 ccaccacttc agagatctgt ccgtactttg agaagacctg ctccagcgac tgctcattgg
      661 tgtcaaaact cagccctcca acaaaaagtt tgccttcatc tgatgccatg ccaacttttt
      721 tgtacaaact tgtcggaccc tggaagagca cctccagctt gtatagctcg tccatgccga
      781 gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg
      841 ggtctttgct cagggcggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggg
      901 ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg
//
