LOCUS       sequence-120126-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of firefly luciferase in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Stringer BW, Day BW, D'Souza RCJ, Jamieson PR, Ensbey KS, Bruce ZC, 
            Lim YC, Goasdoue K, Offenhauser C, Akgul S, Allan S, Robertson T, 
            Lucas P, Tollesson G, Campbell S, Winter C, Do H, Dobrovic A, Inglis
            PL, Jeffree RL, Johns TG, Boyd AW
  TITLE     A reference collection of patient-derived cell line and xenograft 
            models of proneural, classical and mesenchymal glioblastoma.
  JOURNAL   Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z.
  PUBMED    30894629
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-03-20"; volume: "9(1):4902. doi"; pages: " 
            10.1038/s41598-019-41277-z"
COMMENT     Sequence Label: pF CAG luc hygro
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        118..617
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(153..176)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     550..569
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca
       61 gagagatcct cataaaggcc aagaagggcg gaaagtccaa attgtaagct agctaccggg
      121 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
      181 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
      241 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
      301 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
      361 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
      421 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
      481 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
      541 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
      601 catctccggg cctttcgacc tgcatcccgc caccatgaaa aagcctgaac tcaccgcgac
      661 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
      721 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
      781 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
//
