LOCUS       sequence-120305-         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of 1-353 amino acids of ZFP809 tagged with 
            Flag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Wolf D, Goff SP
  TITLE     Embryonic stem cells use ZFP809 to silence retroviral DNAs.
  JOURNAL   Nature. 2009 Apr 30;458(7242):1201-4. doi: 10.1038/nature07844. Epub
            2009 Mar 8.
  PUBMED    19270682
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07844"; journalName: "Nature"; date: "2009-04-30- 
            30"; volume: "458"; issue: "7242"; pages: "1201-4"
COMMENT     Sequence Label: pcDNA3.1-Flag-ZFP809(1-353)
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             91..156
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcatg gactacaaag accatgacgg tgattataaa
      121 gatcatgaca tcgattacaa ggatgacgat gacaagaagc ttggtaccga gctcggatcc
      181 accatggggt tggtgtcctt tgaggatgta gccgtggact tcaccttgga ggagtggcag
      241 gacctggacg ctgctcagag gaccctgtac agggatgtga tgctggaaac ctacagcagc
      301 ctggtgttcc tggacccctg cattgccaaa cctaagttga tcttcaattt ggagcgtgga
      361 tttgggccat ggagcctagc agaagcttca agcaggagcc tcccaggtgt ccataacgtg
      421 agtactctga gtgacaccag caagaaaatt cctaagacac gtttgcggca actcagaaaa
      481 actaaccaaa agacaccaag tgaagacacg attgaagcag aactaaaggc tcgacaggaa
      541 gtctccaaag ggacaacatc ccgtcataga agagcccccg taaaatcttt gtgccgcaag
      601 tcacggagaa ccaagaatca gacatcatac aatgatggga atctctatga atgtaaggac
      661 tgcgagaaag ttttctgtaa taattcaacc ctaattaagc attacagaag aactcataat
      721 gtgtacaagc cctatgagtg tgatgaatgc agtaaaatgt actattggaa gtcagacc
//
