LOCUS       sequence-120380-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, C1 Cloning Vector, Excitation:505 / 569, 
            Emission:516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4a-CenpA-C-22
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..714
                     /codon_start=1
                     /product="fixation-resistant green-to-red photoswitchable 
                     fluorescent protein (Paez-Segala et al., 2015)"
                     /label=mEos4a
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICYA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 ccgtcagatc cgctagcgct accggtcgcc accatggtga gtgcgattaa gccagacatg
       61 aggatcaaac tccgtatgga aggcaacgta aacgggcacc actttgtgat cgacggagat
      121 ggtacaggca agccttatga gggaaaacag accatggatc ttgaagtcaa agagggcgga
      181 cctctgcctt ttgcctttga tatcctgacc actgcattcc attacggcaa cagggtattc
      241 gttaaatatc cagacaacat acaagactat tttaagcagt cgtttcctaa ggggtattcg
      301 tgggaacgaa gcttgacttt cgaagacggg ggcatttgct atgccagaaa cgacataaca
      361 atggaagggg acactttcta taataaagtt cgattttatg gtaccaactt tcccgccaat
      421 ggtccagtta tgcagaagaa gacgctgaaa tgggagccct ccactgagaa aatgtatgtg
      481 cgtgatggag tgctgacggg tgatattcat atggctttgt tgcttgaagg aaatgcccat
      541 taccgatgtg acttcagaac tacttacaaa gctaaggaga agggtgtcaa gttaccaggc
      601 taccactttg tggaccacgc cattgagatt ttaagccatg acaaagatta caacaaggtt
      661 aagctgtatg agcatgctgt tgctcattct ggattgcctg acaatgccag acgatccgga
      721 ctcagatctc gagctcaagc ttcgaattct agcagttcta gagtaagcag cagagtaagt
      781 ggcccgcgcc gccggagccg aaagcccgag gccccgagga ggcg
//
