LOCUS       sequence-120388-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytosolic FRET, Excitation: , Emission: .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Ai HW, Hazelwood KL, Davidson MW, Campbell RE
  TITLE     Fluorescent protein FRET pairs for ratiometric imaging of dual 
            biosensors.
  JOURNAL   Nat Methods. 2008 May;5(5):401-3. doi: 10.1038/nmeth.1207.
  PUBMED    18425137
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2008-05"; volume: "5"; issue: 
            "5"; pages: "401-3"
COMMENT     Sequence Label: mAmetrine-mStrawberry-FRET
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(63..65,66..68,69..779)
                     /codon_start=1
                     /product="yellow fluorescent protein with a large Stokes 
                     shift, excitable with violet light (Ai et al., 2008)"
                     /label=mAmetrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTSGKLPVPWPTLVTTLSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GSYRTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNMNVWDAYITADKQKNGIK
                     ANFKIEHNVEDGGVQLADAYQQNTPIGDGSVLLPDNHYLSFQSKLFKDPNEQRDHMVLL
                     EFVTAAGITPGMDELYK"
     primer_bind     complement(108..129)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tgaaccgtca gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattca
       61 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      121 acggcgacgt aaacggccac aagttcagcg tgcgcggcga gggcgagggc gatgccacca
      181 acggcaagct gaccctgaag ttcatctgca cctccggcaa gctgcccgtg ccctggccca
      241 ccctcgtgac caccctgtct tacggcgtgc agtgcttcgc ccgctacccc gaccacatga
      301 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      361 ccttcaagga cgacggcagc tacaggaccc gcgccgaggt gaagttcgag ggcgacaccc
      421 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
      481 acaagctgga gtacaacatg aacgtgtggg acgcgtatat cacggccgac aagcagaaga
      541 acggcatcaa agcgaacttc aagatcgagc acaacgtcga ggacggcggc gtgcagctcg
      601 ccgacgcgta ccagcagaac acccccatcg gcgacggctc cgtgctgctg cctgacaacc
      661 actacctgag cttccagagc aagctgttca aagaccccaa cgagcagcgc gatcacatgg
      721 tcctgctgga gttcgttacc gccgccggga tcactcccgg catggacgag ctgtacaagt
      781 ccggactcag atccccaccg gtggctacca tggtgagcaa gggc
//
