LOCUS       sequence-120407-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of P. leioghnati LUMP protein, a FRET acceptor 
            protein emitting a cyan-colored fluorescence with an unusually long 
            excited state lifetime.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Hoepker AC, Wang A, Le Marois A, Suhling K, Yan Y, Marriott G
  TITLE     Genetically encoded sensors of protein hydrodynamics and molecular 
            proximity.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 30. pii: 201424021.
  PUBMED    25931526
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Apr 30. pii: 201424021."
COMMENT     Sequence Label: 130_pSKB3-LUMP-P.leioghnati
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             83..103
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             683..700
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(753..771)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cgattacgat atcccaacga ccgaaaacct ttacttccag ggccatatgt ttagaggtat
      121 tgttcaaggt cgtggtgtta ttcgatccat ttcaaaaagt gaagatagtc agcgacatgg
      181 gattgcattc cctgagggta tgtttcaact tgttgatgta gatactgtaa tgttagtaaa
      241 tggttgttca ttgacagttg tccgtatttt aggcgatatg gtgtactttg atatcgacca
      301 agcattgggt acgacaacat ttgatggatt aaaagaaggc gatcaggtaa acctagagat
      361 ccaccctaaa tttggtgaag tggttggccg tggtggatta acaggaaata ttaaaggcac
      421 tgcgctggtc gcagcaatag aagagaatga cgcaggtttt tctgtactga ttgatatccc
      481 taaagggtta gctgaaaact taacagtgaa agatgatatt ggaattgatg ggatctcttt
      541 gcctatcact gatatgtcag acagcattat tacattgaat tactcgcgtg atttgttagc
      601 gagtactaac attgcatcgt tagcaaaaga tgtgaaagtg aacgttgaaa ttttaaatga
      661 atggtaggcg gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa
      721 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
      781 tgggg
//
