LOCUS       sequence-120461-         453 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 CIRBP
FEATURES             Location/Qualifiers
     source          1..453
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..45
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    46..70
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggcatggacg agctatacaa gctggaggtg ctcttccagg gtccgacaag tttgtacaaa
       61 aaagttggca tggcatcaga tgaaggcaaa ctttttgttg gagggctgag ttttgacacc
      121 aatgagcagt cgctggagca ggtcttctca aagtacggac agatctctga agtggtggtt
      181 gtgaaagaca gggagaccca gagatctcgg ggatttgggt ttgtcacctt tgagaacatt
      241 gacgacgcta aggatgccat gatggccatg aatgggaagt ctgtagatgg acggcagatc
      301 cgagtagacc aggcaggcaa gtcgtcagac aaccgatccc gtgggtaccg tggtggctct
      361 gccgggggcc ggggcttctt ccgtgggggc cgaggacggg gccgtgggtt ctctagagga
      421 ggaggggacc gaggctatgg ggggaaccgg ttc
//
