LOCUS       sequence-120465-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 IL6
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..36
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(698..722)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagctataca agctggaggt gctcttccag ggtccgacaa gtttgtacaa aaaagttggc
       61 atgaactcct tctccacaag cgccttcggt ccagttgcct tctccctggg gctgctcctg
      121 gtgttgcctg ctgccttccc tgccccagta cccccaggag aagattccaa agatgtagcc
      181 gccccacaca gacagccact cacctcttca gaacgaattg acaaacaaat tcggtacatc
      241 ctcgacggca tctcagccct gagaaaggag acatgtaaca agagtaacat gtgtgaaagc
      301 agcaaagagg cactggcaga aaacaacctg aaccttccaa agatggctga aaaagatgga
      361 tgcttccaat ctggattcaa tgaggagact tgcctggtga aaatcatcac tggtcttttg
      421 gagtttgagg tatacctaga gtacctccag aacagatttg agagtagtga ggaacaagcc
      481 agagctgtgc agatgagtac aaaagtcctg atccagttcc tgcagaaaaa ggcaaagaat
      541 ctagatgcaa taaccacccc tgacccaacc acaaatgcca gcctgctgac gaagctgcag
      601 gcacagaacc agtggctgca ggacatgaca actcatctca ttctgcgcag ctttaaggag
      661 ttcctgcagt ccagcctgag ggctcttcgg cagatgtacc caactttctt gtacaaagtg
      721 gtgtaagcgg ccgccctcct cctcctttct tgttcctttc acgtcgcctt ctcggttgta
      781 gctgg
//
