LOCUS       sequence-120466-         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 TNF
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..36
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(761..785)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagctataca agctggaggt gctcttccag ggtccgacaa gtttgtacaa aaaagttggc
       61 atgatcactg aaagcatgat ccgggacgtg gagctggccg aggaggcgct ccccaagaag
      121 acaggggggc cccagggctc caggcggtgc ttgttcctca gcctcttctc cttcctgatc
      181 gtggcaggcg ccaccacgct cttctgcctg ctgcactttg gagtgatcgg cccccagagg
      241 gaagagttcc ccagggacct ctctctaatc agccctctgg cccaggcagt cagatcatct
      301 tctcgaaccc cgagtgacaa gcctgtagcc catgttgtag caaaccctca agctgagggg
      361 cagctccagt ggctgaaccg ccgggccaat gccctcctgg ccaatggcgt ggagctgaga
      421 gataaccagc tggtggtgcc atcagagggc ctgtacctca tctactccca ggtcctcttc
      481 aagggccaag gctgcccctc cacccatgtg ctcctcaccc acaccatcag ccgcatcgcc
      541 gtctcctacc agaccaaggt caacctcctc tctgccatca agagcccctg ccagagggag
      601 accccagagg gggctgaggc caagccctgg tatgagccca tctatctggg aggggtcttc
      661 cagctggaga agggtgaccg actcagcgct gagatcaatc ggcccgacta tctcgacttt
      721 gccgagtctg ggcaggtcta ctttgggatc attgccctgt gcccaacttt cttgtacaaa
      781 gtggtgtaag cggccgccct cctcctcctt tcttgttcct ttcacgtcgc cttctcggtt
      841 gtagctggca gacgacgagt cttactttta cgtgtacttc tctatagatg atgtatgatc
      901 tctctgcatg cgtgttcgtg catgtgtccg tgtgttgggt acgcgtggta ccctgc
//
