LOCUS       sequence-120516-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Complementation in Cryptococcus neoformans. Targets DNA constructs 
            to a Safe Haven Site, a small gene-free region. Contains NEO 
            resistance marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Arras SD, Chitty JL, Blake KL, Schulz BL, Fraser JA
  TITLE     A genomic safe haven for mutant complementation in Cryptococcus 
            neoformans.
  JOURNAL   PLoS One. 2015 Apr 9;10(4):e0122916. doi: 
            10.1371/journal.pone.0122916. eCollection 2015.
  PUBMED    25856300
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-9"; volume: "10(4):e0122916. doi"; pages: " 
            10.1371/journal.pone.0122916. eCollection 2015"
COMMENT     Sequence Label: pSDMA57
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     277..296
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
       61 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
      121 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
      181 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
      241 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
      301 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
      361 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagtg aaggcggtaa
      421 ggggttaatt ttccttagag ggtgtatata tacatattag agaagtgata caattttagc
      481 acactgcgaa ttcgagacag acatcgtgtc aatcatcttt tttgacatta tatgcccatt
      541 aatctatcta cagacaacaa taccatcctt cccaccctca gcaacgccgt tgaatcctca
      601 ggatcttcat ggctccttgt ctctgaaacc aggaagctag tttctacatc tcttccgtgt
      661 taatacagat aaaccggcca atgaagcacg ctcaaaatta tgcctgtcaa aacagtcagc
      721 tccatccttt tcttcccttt acgtatcata tgctgttttt tttttttttt cgacacaagt
      781 tcaaagggct ggcgctgctt aggatccatc acaccattcg tcaacgtatt gtgaatcaaa
      841 gaaatgctct tcgatattct ttatcagggc aagggaacag acagatacca gcaggcttca
      901 caactctgag tcgctcggcc aaagaggaaa agaaaagagg atgccggcac tttctatac
//
