LOCUS       sequence-120592-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 RAD54B
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gtatgtgcca gtagcttgag tggttatatt ctgaaaaatg aatgacacat
      241 tttctgttat tctttcaaga aaaggatctg taagatttaa atgatctcca gaaaaatgtt
      301 tccattgctt cagctgggac atagaaagag gtttcaggga gttagatttc tggtgatgtg
      361 gaccaagctg acaatctcta gagacaatga atttttccaa cgaatcacct gtatgaactt
      421 cttctcctgt acactcacag tcaagcagat catgagtaac acaatctgaa ctttcatgta
      481 atgtgaacaa atttttaagt tcttctactg aaaactgaat atgttcagat gtcttggtga
      541 ggtcgacaac tgccccacaa agaccttgct tactgatctg cctttgatag atcttttctt
      601 ctattgtacc tgtagttagg agtctgtaaa tatgtacagg atatttctga ccatctctcc
      661 atactctaga cattgcctga atgtcagtgg ctggattcca atcaatgtca tagagaatta
      721 agtgagatcc tccaatgagg ttaagtccta caccaccagc ttttgaactt aacaaaaaaa
      781 taaaaaaaga agagtgttga ctgttaaagc catcaacaat ctgctgcctt tgagagattg
      841 gtgtttgtcc atcaagtctt gtataagcat atccatgacg cttacatact tcttgtaaaa
//
