LOCUS       sequence-120632-        7722 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length P. vivax P41 ectodomain in mammalian cells. 
            C-terminal rat Cd4d3+4 tag, biotinylation sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7722)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 7722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2015-12-23"; volume: "9(12):e0004264. doi"; 
            pages: " 10.1371/journal.pntd.0004264. eCollection 2015 Dec"
COMMENT     Sequence Label: P41-bio
FEATURES             Location/Qualifiers
     source          1..7722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3102..3157
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3156..3175)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5393..5411)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5479..5583
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5584..5652,5653..6444)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5802..5821)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6615..7203
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7104..7123
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7357..7374
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7491..7512
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7527..7532,7533..7550,7551..7557)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7565..7581
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7570..7592
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7589..7605
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7589..7605
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgcagagg aacacatctg cgacttcacc aaagagaagt
     1381 acctgctggg caagaacgag aaagaatact gcgtcgtgaa cgccaagccc ttcgacagcg
     1441 tgaccttcat ctgccccaag aaaatcggcg cccagtgctt ccagaacgtg aacaccctgg
     1501 acgacatcag cgccgacaag atggaaagca gcaagctgag catcgacgtg ctgctgtacg
     1561 gctccaccct gtacggcgat accctgctga tctcccccac cgtgaagcag tctaccacct
     1621 tctactgttt ctgcaacctg cagatggaag atctgaagaa atacctgaag aagcggcggc
     1681 tgacaaaaga gaaagagaac gccaaaaaga aaagcaccgt gaatgtgaac gacctgaaaa
     1741 acgccgacga ggacatggaa gtggtggtgc ccgagaagca gatcgacgag catctcgtgc
     1801 gggccctgta cagagtgaag aagatccgga acatcatcga gcgcgagaag aacaagggcg
     1861 agggcgacaa gcccaccaac cccgaggatg aagaggaact cgtgatcgag gaagaacagg
     1921 aagaagagga cggcgaaggc gacgaagagg atgagagcaa ggtggaaaag atcatcacca
     1981 aatacggcat catgaaggtg gtggtgtcca ccaacaacgc catcaccaag ggctgcgatt
     2041 tcggcaacaa cgtcgtgaat tacttcagca agccctaccc cgtggaaaga tacggcggca
     2101 gcaaagtgtg ccggatcgag gccaagcctg gcgagttcgt gggcttcaag tgcatctacg
     2161 acaaccaggg caccgtggaa ccccacaact gcttcgacaa ggtgttctac gagggcaacg
     2221 aggccgacct gcagaccctg atgcctggct acatcagcta cggcaacaag cagaagggga
     2281 agtacgcctt ctacctgaag ctgccccact tcgtgcagca cagctacacc gtgcagtgca
     2341 agtgccggtc caccgtgcac cagtacgacg actacgtgtt cgagctggcc gtggaaggcg
     2401 gcgagtctga catcgtggcc aagagctttc aggaaggcgc gccgtcgacc tccatcacgg
     2461 cctataagag tgagggggag tcagcggagt tctccttccc actcaacctt ggagaggaaa
     2521 gcctgcaggg agagttgaga tggaaggcag agaaggctcc ttcttcccag tcctggatca
     2581 ccttctccct aaagaaccaa aaggtgtctg tgcagaagtc tactagcaac cccaagttcc
     2641 agctgtccga aacgctccca ctcacccttc agatacccca ggtctccctt cagtttgctg
     2701 gttctggcaa cctgaccctg actctggaca gagggatact gtatcaggaa gtgaacctgg
     2761 tggtgatgaa agtgactcag cccgacagca acactttgac ctgtgaggtg atgggaccca
     2821 cctcacccaa gatgagactg atcttgaagc aggagaatca ggaggccagg gtctccaggc
     2881 aggagaaagt gattcaagtg caggcccctg aagcaggggt gtggcaatgt ctactgagtg
     2941 aaggtgaaga ggtcaagatg gactccaaga tccaggtttt atccaaaggg ttgaattccg
     3001 gatcactgca tcatattctg gatgcacaga aaatggtgtg gaatcatcgt taagtctaga
     3061 cacgtgatta attaaggatc ccccgacctc gacctctggc taataaagga aatttatttt
     3121 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     3181 aaatcatttg gtcgagatcc ctcggagatc tctagctaga ggatcgatcc ccgccccgga
     3241 cgaactaaac ctgactacga catctctgcc ccttcttcgc ggggcagtgc atgtaatccc
     3301 ttcagttggt tggtacaact tgccaactgg gccctgttcc acatgtgaca cgggggggga
     3361 ccaaacacaa aggggttctc tgactgtagt tgacatcctt ataaatggat gtgcacattt
     3421 gccaacactg agtggctttc atcctggagc agactttgca gtctgtggac tgcaacacaa
     3481 cattgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg ggacatgtac
     3541 ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg
     3601 tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata aggccccctt
     3661 gttaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat ccagactaac
     3721 cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt agggttagta
     3781 aaagggtcct aaggaacagc gatatctccc accccatgag ctgtcacggt tttatttaca
     3841 tggggtcagg attccacgag ggtagtgaac cattttagtc acaagggcag tggctgaaga
     3901 tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc tccttcgttt
     3961 agctaataga ataactgctg agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca
     4021 aggtttcagg tgacgccccc agaataaaat ttggacgggg ggttcagtgg tggcattgtg
     4081 ctatgacacc aatataaccc tcacaaaccc cttgggcaat aaatactagt gtaggaatga
     4141 aacattctga atatctttaa caatagaaat ccatggggtg gggacaagcc gtaaagactg
     4201 gatgtccatc tcacacgaat ttatggctat gggcaacaca taatcctagt gcaatatgat
     4261 actggggtta ttaagatgtg tcccaggcag ggaccaagac aggtgaacca tgttgttaca
     4321 ctctatttgt aacaagggga aagagagtgg acgccgacag cagcggactc cactggttgt
     4381 ctctaacacc cccgaaaatt aaacggggct ccacgccaat ggggcccata aacaaagaca
     4441 agtggccact cttttttttg aaattgtgga gtgggggcac gcgtcagccc ccacacgccg
     4501 ccctgcggtt ttggactgta aaataagggt gtaataactt ggctgattgt aaccccgcta
     4561 accactgcgg tcaaaccact tgcccacaaa accactaatg gcaccccggg gaatacctgc
     4621 ataagtaggt gggcgggcca agataggggc gcgattgctg cgatctggag gacaaattac
     4681 acacacttgc gcctgagcgc caagcacagg gttgttggtc ctcatattca cgaggtcgct
     4741 gagagcacgg tgggctaatg ttgccatggg tagcatatac tacccaaata tctggatagc
     4801 atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc
     4861 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc
     4921 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     4981 atatgctatc ctaatctgta tccgggtagc atatgctatc ctaatagaga ttagggtagt
     5041 atatgctatc ctaatttata tctgggtagc atatactacc caaatatctg gatagcatat
     5101 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagcatag
     5161 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     5221 gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     5281 gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat
     5341 gctatcctca tgataagctg tcaaacatga gaattttctt gaagacgaaa gggcctcgtg
     5401 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     5461 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     5521 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     5581 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     5641 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     5701 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     5761 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     5821 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     5881 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5941 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     6001 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     6061 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     6121 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     6181 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     6241 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     6301 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     6361 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     6421 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     6481 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6541 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6601 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6661 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6721 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     6781 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6841 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6901 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6961 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     7021 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7081 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7141 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     7201 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     7261 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     7321 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     7381 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     7441 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     7501 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     7561 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     7621 ctctagctag aggtcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa
     7681 cgttgttgcc attgctgcag gcgcagaact ggtaggtatg ga
//
