LOCUS       sequence-120638-        8820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length P. vivax GAMA ectodomain in mammalian cells. 
            C-terminal rat Cd4d3+4 tag, biotinylation sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8820)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 8820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2015-12-23"; volume: "9(12):e0004264. doi"; 
            pages: " 10.1371/journal.pntd.0004264. eCollection 2015 Dec"
COMMENT     Sequence Label: GAMA-bio
FEATURES             Location/Qualifiers
     source          1..8820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4200..4255
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4254..4273)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6491..6509)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6577..6681
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6682..6750,6751..7542)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6900..6919)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7713..8301
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8202..8221
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8455..8472
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8589..8610
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8625..8630,8631..8648,8649..8655)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8663..8679
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8668..8690
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8687..8703
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8687..8703
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccctga tcagaaacgg caacaatcct caggccctgg
     1381 tgcccgagaa aggcgccgat ccttctggcg gacagaacaa cagagccggc gagaaccagg
     1441 atacctgcga gatccagaaa atggccgaag agatgatgga aaagatgatg aaagaaaagg
     1501 acgtgttcag cagcatcatg gaacccctgc agagcaagct gaccgacgac cacctgtgca
     1561 gcaagatgaa gtacaccaac atctgcctgc acgagaagga caagaccccc ctgaccttcc
     1621 catgcaccag cccccagtac gagcagctga tccaccggtt cacctacaag aagctgtgca
     1681 acagcaaggt ggccttcagc aacgtgctgc tgaagtcctt catcgacaag aagaacgagg
     1741 aaaacacctt caacaccatc atccagaact acaaggtgct gagcacatgc atcgacgacg
     1801 acctgaagga catctacaac gccgccatcg agctgttcag cgacatccgg accagcgtga
     1861 ccgagatcac cgagaaactg tggtccaaga acatgatcga ggtgctgaaa acccgcgagc
     1921 agacaatcgc cggcatcctg tgcgagctgc ggaatggcaa caacgccccc ctggtgtcca
     1981 acagcttcag ctacgagaac ttcgggatcc tgaaagtgaa ctacgagggc ctgctgaacc
     2041 aggcctacgc cgccttcagc gactactaca gctacttccc cgccttcgcc atctccatgc
     2101 tggaaaaggg cggcctggtg gacagactgg tggccatcca cgagagcctg accaactacc
     2161 ggacccggaa catcctgaag aagatcaacg agaagtccaa gaatgaagtg ctgaacaacg
     2221 aagagatcat gcacagcctg agcagctaca agcaccacgc tggcggaacc agaggcgcct
     2281 tcctgcagtc tagagatgtg cgggaagtga cccagggcga cgtgtccgtg gatgagaagg
     2341 gcgatagagc caccacagcc ggcggaaatc aggccgcttc tgtggctgcc gccgctccta
     2401 aagatgccgg ccctacagtg gccgctccaa atactgccgc caccctgaaa acagccgcca
     2461 gccctaatgc cgctgccacc aatactgctg ccccccctaa tatgggcgcc accagccctc
     2521 tgagcaaccc cctgtacggc acaagcagcc tgcagcccaa agacgtggca gtgctcgtgc
     2581 gggacctgct gaagaacacc aatatcatca agttcgagaa caatgagccc accagccaga
     2641 tggacgacga ggaaatcaag aaactgatcg agagcagctt cttcgacctg agcgacaaca
     2701 ccatgctgat gcggctgctg atcaagcctc aggccgccat cctgctgatt atcgagagct
     2761 ttatcatgat gacccccagc cccacccggg acgccaagac ctactgtaaa aaagccctcg
     2821 tgaacggcca gctgatcgaa accagcgacc tgaatgccgc cactgaagag gacgatctga
     2881 tcaatgagtt cagctcccgg tacaacctgt tctacgagag actgaagctg gaagaactgc
     2941 gcgagatcga gcagaaccgg aaggccctga agaatagcaa gggcaccctg agcgtgctgg
     3001 aagtggccaa cagccagaac gcccctgatg gcaagggcgt gaacggggct ggcaatgccg
     3061 ctaatgccaa cgccgcaaac gccaacgctg ccaatgccaa tgcagctaac gctaatgcag
     3121 ccaacggcaa cctggccaac gccaatctgg caaatgcaaa cgctgctaac gccgatgccg
     3181 ccaacgcaaa tgccgccaat gctaacgcag ccaatgcaaa cgccgccaac gctaatctgg
     3241 ccaacgctaa cctggctaat gccaacctgg caaacgccaa cctggctaac gctaatctgg
     3301 ccaatgccaa tctggccaac gccaatgccg caaatgccaa tgctgccaac gctaacgccg
     3361 ctaacggcaa cgcccccaac agcaacaatg gcgccggaag cccactgatc gtggtcgtgg
     3421 gagccgacct gggcgagaaa accgaggaca tcatcaagaa caacgtggac gtggccgcac
     3481 tgaccgccga tgtggaacag gccttcaaga acctggaact gcagagcgga tctggcgcgc
     3541 cgtcgacctc catcacggcc tataagagtg agggggagtc agcggagttc tccttcccac
     3601 tcaaccttgg agaggaaagc ctgcagggag agttgagatg gaaggcagag aaggctcctt
     3661 cttcccagtc ctggatcacc ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta
     3721 ctagcaaccc caagttccag ctgtccgaaa cgctcccact cacccttcag ataccccagg
     3781 tctcccttca gtttgctggt tctggcaacc tgaccctgac tctggacaga gggatactgt
     3841 atcaggaagt gaacctggtg gtgatgaaag tgactcagcc cgacagcaac actttgacct
     3901 gtgaggtgat gggacccacc tcacccaaga tgagactgat cttgaagcag gagaatcagg
     3961 aggccagggt ctccaggcag gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt
     4021 ggcaatgtct actgagtgaa ggtgaagagg tcaagatgga ctccaagatc caggttttat
     4081 ccaaagggtt gaattccgga tcactgcatc atattctgga tgcacagaaa atggtgtgga
     4141 atcatcgtta agtctagaca cgtgattaat taaggatccc ccgacctcga cctctggcta
     4201 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
     4261 aaggacatat gggagggcaa atcatttggt cgagatccct cggagatctc tagctagagg
     4321 atcgatcccc gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg
     4381 ggcagtgcat gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac
     4441 atgtgacacg gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat
     4501 aaatggatgt gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt
     4561 ctgtggactg caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca
     4621 atgctggggg acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt
     4681 caatcagagg ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag
     4741 tgtttataag gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta
     4801 tatactatcc agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct
     4861 atcgaattag ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct
     4921 gtcacggttt tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac
     4981 aagggcagtg gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt
     5041 cttcattctc cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat
     5101 gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg
     5161 ttcagtggtg gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa
     5221 atactagtgt aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg
     5281 gacaagccgt aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata
     5341 atcctagtgc aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag
     5401 gtgaaccatg ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca
     5461 gcggactcca ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg
     5521 ggcccataaa caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc
     5581 gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg
     5641 ctgattgtaa ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc
     5701 accccgggga atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg
     5761 atctggagga caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct
     5821 catattcacg aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta
     5881 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat aggctatcct
     5941 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     6001 aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     6061 aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct
     6121 aatagagatt agggtagtat atgctatcct aatttatatc tgggtagcat atactaccca
     6181 aatatctgga tagcatatgc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     6241 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     6301 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc
     6361 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc
     6421 tgtatccggg tagcatatgc tatcctcatg ataagctgtc aaacatgaga attttcttga
     6481 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     6541 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     6601 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     6661 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     6721 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     6781 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     6841 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     6901 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     6961 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     7021 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     7081 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     7141 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     7201 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     7261 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     7321 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     7381 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     7441 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     7501 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     7561 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     7621 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7681 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     7741 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7801 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     7861 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7921 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7981 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     8041 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     8101 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     8161 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     8221 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     8281 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     8341 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     8401 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     8461 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     8521 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     8581 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     8641 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     8701 gaccatgatt acgccaagct ctagctagag gtcgaccaat tctcatgttt gacagcttat
     8761 catcgcagat ccgggcaacg ttgttgccat tgctgcaggc gcagaactgg taggtatgga
//
