LOCUS       sequence-120744-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 TYK2
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    165..189
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccacgcggta cccaacacac ggacacatgc acgaacacgc atgcagagag atcatacatc
       61 atctatagag aagtacacgt aaaagtaaga ctcgtcgtct gccagctaca accgagaagg
      121 cgacgtgaaa ggaacaagaa aggaggagga gggcggccgc ttacaccact ttgtacaaga
      181 aagttgggca ggcacacgct gaacactgaa ggggcctggc cttggtactt ctcatggact
      241 gtcttcagaa tgggtatgag gttctcgaag gttgggcgaa aggacgcctc tgtctcccag
      301 cagttcttca tgagatgata gacctcacag ggacatttgt cgggccgtgg cagcctctcc
      361 cctcgttcca gcaactcagt gagtctcaga actgtcatct gaccctgagc aatgcctatg
      421 agctcaagga atttcgtggg ggggctctgg ctggagtcac agtgcgtcag cagctcatac
      481 agggtcaccc cgaaggacca gacatctgac gcatagtaga acttatactc cttcaggcac
      541 tctggggcat accagaacac ggggctgtcc ccatcctcgc gcacgcggta gtactcgtgg
      601 ccttcgggca cggccttggc taggccaaag tccccgatct tgaccagcct gtcgttgtcc
      661 agcagcacgt tgcgcgcggc taggtctcgg tggatgtagt gctgcgagtg cagataggcc
      721 atgccctcgc agatctgctg ggcgaagagc agcagctggg ccagcccgat gctgtgccgg
      781 ggcaggtagt ctcggaggct gcccaggggc acgtactcca tgaccagctg cagcgacttc
      841 tcgccttggt cctcgcagca gcccttgtac ttgatgatgt gctcgtggta gagcgtgcgc
//
